TORCH感染对人胎盘滋养细胞的影响及机制  

Effect of TORCH infection on placental trophoblastic cells and the mechanism

作　　者：蔡旺[1] 王海丽[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《中国妇幼保健》2016年第10期2180-2183,共4页Maternal and Child Health Care of China

基　　金：辽宁省科技厅自然科学基金项目(2013022034)

摘　　要：目的研究TORCH感染对体外培养人胎盘滋养细胞增殖、凋亡的影响及其分子机制。方法采用复合酶消化法和Percoll密度梯度离心法分离纯化人胚胎滋养细胞,实验组采用TORCH即弓形虫(Tox)、风疹病毒(RV)、巨细胞病毒(CMV)和单纯疱疹病毒(HSV)分别感染人胎盘滋养细胞,对照组加入DMEM高糖培养基孵育;在24、48、72 h用MTT法检测细胞抑制率,应用流式细胞术分析TORCH感染对体外培养人胎盘滋养细胞凋亡及周期分布的影响以及免疫印迹法检测细胞分裂周期蛋白-6(Cell division cycle-6,Cdc-6)的表达水平。结果 TORCH感染体外培养的人胎盘滋养细胞后,实验组人胎盘滋养细胞增殖明显受到抑制并与感染时间相关,人胎盘滋养细胞凋亡率也显著增高,细胞周期主要表现为G1期细胞比例增加,S期细胞比例降低。同时,Cdc-6蛋白的表达水平明显降低。结论 TORCH感染可以抑制人胎盘滋养细胞增殖、使人胎盘滋养细胞停滞在G1期诱导人胎盘滋养细胞凋亡,其可能与抑制Cdc-6蛋白的表达有关。Objective To study the effect of TORCH infection on proliferation and apoptosis of placental trophoblastic cells in vitro and the mechanism. Methods Placental trophoblastic cells were isolated and purified using compound enzymes digestion and Percoll density gradient centrifugation. In experimental group,placental trophoblastic cells were infected by TORCH including Toxoplasma gondii（ Tox）,Rubella virus（ RV）,Cytomegalovirus（ CMV） and Herpes simplex（ HSV）,respectively; in control group,placental trophoblastic cells were incubated in DMEM medium supplemented by high glucose. The inhibition rates of placental trophoblastic cells at 24,48,and 72 hours were determined using MTT assay,and the effect on cell apoptosis and cell cycle distribution of TORCH-infected placental trophoblastic cells were analyzed using flow cytometry（ FCM）,and the expression level of cell division cycle- 6（ Cdc- 6） was detected by western blot.Results After TORCH infection,the proliferation of placental trophoblastic cells in experimental group was inhibited in a time dependent manner,cell apoptostic rate also increased significantly; the cell cycle showed increased percentage of G1 stage cells and decreased percentage of S stage cells. Meanwhile,the expression level of Cdc-6 protein decreased significantly. Conclusion TORCH infection suppresses proliferation of placental trophoblastic cells,induces cell apoptosis through retarding placental trophoblastic cells in G1 stage,which may be related to the expression of Cdc-6 protein.

关 键 词：TORCH 人胎盘滋养细胞 增殖 细胞分裂周期蛋白-6 

分 类 号：R714.25[医药卫生—妇产科学]