应用于大分子药物经皮释药的可溶微针贴片制备  

The preparation of the dissolving microneedle array of macromolecular drugs through percutaneous release

作　　者：王清清[1] 马涛[1] 李见春[1] 王秀[1] 张鑫宇[1] 刘浩[1] 吴传斌[2] 

机构地区：[1]蚌埠医学院药学系,安徽蚌埠233030 [2]中山大学药学院,广东广州510006

出　　处：《蚌埠医学院学报》2016年第4期523-527,共5页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81502994);安徽省自然科学基金项目(1608085QH179);蚌埠市科技发展指导性项目(20150331);蚌埠医学院开放课题计划(BYKC1402ZD)

摘　　要：目的:建立可溶微针贴片(DMNA)的制备方法,为大分子药物经皮释药研究提供基础。方法:采用计算机辅助微机械加工技术制备微针主模,利用聚二甲基硅氧烷(PDMS)材料制备阴模,通过扫描电子显微镜(SEM)对阴模进行表征。以卡波普为辅料进行制备方法研究与优化,利用离体大鼠皮肤评价优化方法制备的可溶微针的机械性能。结果:自主设计和制造了具有精细结构的微针金属主模,阴模的SEM图片表明主模、阴模尺寸精细,与设计一致;采用优化的离心注模和室温干燥法制备的DMNA,具备与模具一致的外观,微针能成功穿透离体大鼠皮肤,在皮肤内快速溶解。结论:建立了一种新的DMNA制备方法,为进一步大分子药物经皮释药研究提供了基础。Objective： To construct the preparation method of the dissolving microneedle array（ DMNA）,and provide the basis for percutaneous release of macromolecular drugs. Methods： The metal master mold of DMNA was manufactured using computer-assisted micro-machining technique. The female mold was made from polydimethylsiloxane（ PDMS）,and charactered by scanning electron microscope（ SEM）. The preparation process of DMNA was optimized using carbopol as microneedle material. The mechanical properties of the optimized DMNAs were evaluated by using the rat skin in vitro. Results： The microneedle metal master mold was nicety. The SEM image of female mold showed that it was accurate,and consistent with the design. The DMNAs fabricated by optimized method had the same shape as mold,the microneedles can penetrate into the rat skin,and dissolve quickly. Conclusions： The new preparation method of the DMNA can provide the basis for percutaneous release of macromolecular drugs.

关 键 词：给药 皮下 可溶微针贴片 大鼠 

分 类 号：R452[医药卫生—治疗学]