中和白细胞介素-23抑制血管紧张素Ⅱ诱导的动脉粥样硬化进程  

Neutralization of interleukin 23 attenuates angiotensinⅡ-induced atherosclerosis in ApoE-/-mice

作　　者：孟凯[1] 吴帮卫 黄瑛[2] 林英忠[3] 刘伶[3] 俞坤武 曾秋棠[1] 吉庆伟[3] 

机构地区：[1]华中科技大学附属协和医院心内科,武汉430022 [2]广西壮族自治区人民医院超声科 [3]广西壮族自治区人民医院心内科

出　　处：《临床心血管病杂志》2016年第5期509-512,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金资助项目(No:81160045;81270354;81360055;81460081);广西医疗卫生重点科研课题(No:重2011119);广西自然科学基金资助项目(No:2013GXNSFAA019265)

摘　　要：目的:探讨中和白细胞介素(IL)-23是否影响血管紧张素Ⅱ诱导的动脉粥样硬化进程及可能机制。方法:10周龄雄性ApoE-/-小鼠随机分为两组,给予8周西方高脂饮食的同时皮下植入血管紧张素Ⅱ微泵,分别经腹腔注射抗IL-23p19中和抗体和同型对照抗体。8周后处死小鼠,检测血压、心率、斑块面积、Th1和Th17比例、组织和循环炎性因子的表达。结果:抗IL-23p19中和抗体不影响小鼠血压和心率;抗IL-23p19中和抗体处理显著减少斑块面积,降低Th1和Th17比例,下调组织和循环促炎因子干扰素-γ、IL-6、IL-17、肿瘤坏死因子-α水平。结论:中和IL-23通过抑制炎症反应延缓血管紧张素Ⅱ诱导的动脉粥样硬化进程。Objective：To investigate whether neutralization of interleukin 23（IL-23）attenuates angiotensinⅡ（AngⅡ）-induced atherosclerosis in ApoE-/-mice.Method：The 10-week-old male ApoE-/-mice were fed with Western-type diet and implanted osmotic minipumps for the infusion of Ang Ⅱ（8weeks）.The mice were randomly grouped and injected i.p.Once per week for 8week with anti-IL-23p19 antibody or control antibody.Blood pressure,heart rate,atherosclerotic lesion,the frequencies of Th1 and Th17as well as the expression of local and circulating cytokine were measured.Result：No significant difference was found in blood pressure and heart rate in the two groups.Neutralization of IL-23 led to a significant reduction in the atherosclerosis lesion size,the frequencies of Th1 and Th17as well as the production of local and circulating IFN-γ,IL-6,IL-17 and TNF-α.Conclusion：Neutralization of interleukin 23 attenuates angiotensinⅡ-induced atherosclerosis in ApoE-/-mice by inhibiting inflammation.

关 键 词：动脉粥样硬化 血管紧张素Ⅱ IL-23 炎症 

分 类 号：R543[医药卫生—心血管疾病]