猫感染鼠疫菌后F1抗体及其IgM变化与在监测中的应用前景分析  

Dynamics and potential application of F1 antibody and immunoglobulin M in cat after infected with Yersinia pestis

作　　者：王信惠[1] 温娟[1] 王鹏[2] 阿不力米提·买托呼提[1] 热娜·吐尔地[1] 廖力夫[3] 徐秉臣[1] 

机构地区：[1]新疆维吾尔自治区疾病预防控制中心鼠疫防治科,乌鲁木齐830002 [2]云南省地方病防治所中心实验室,大理671000 [3]新疆实验动物中心检测室,乌鲁木齐830002

出　　处：《中华地方病学杂志》2016年第5期316-319,共4页Chinese Journal of Endemiology

基　　金：十一五国家科技支撑计划（2007BA107A14）;新疆高新技术研究发展计划（200611112）

摘　　要：目的观察猫接种鼠疫菌后鼠疫F1抗体及其免疫球蛋白M（IgM）变化，探讨Fl抗体及IgM在动物鼠疫监测中的意义。方法猫3只，在猫背部采用多点皮下注射法接种鼠疫菌，于接种后3～521d，定期股静脉采血，酶联免疫吸附试验（ELISA）夹心法检测鼠疫F1抗体，捕获法检测鼠疫IgM。结果猫接种鼠疫菌后第3天，F1抗体及IgM出现弱阳性反应，平均滴度分别为1：22伽和1：2^0.33。鼠疫F1抗体滴度，第4天升高到1：2^5.33，第97天达到高峰，为1：29”，第521天仍保持在1：2^5.33；IgM滴度，第7～10天出现高峰，为1：2^12.00，其后快速降低，第30天后降到阳性反应标准以下。结论ELISA夹心法检测猫鼠疫F1抗体，可追溯较长时间的动物鼠疫流行，适用于鼠疫疫源地调查；捕获法检测猫鼠疫IgM，单份血清可判定早期动物鼠疫。两种方法同步检测F1抗体及IgM，判定动物感染鼠疫的时间可精确到3～7d。Objective To observe the dynamics of F1 antibody and immunoglobulin M （IgM） in cats after vaccinated Yersinia pestis, to discuss the significance of detection of F1 antibody and IgM in surveillance of animal plague. Methods The 3 cats were vaccinated Yersinia pestis on their backs with muhipoint hypodermic injection and blood samples were collected via femoral vein on 3rd to 521st days after injection. F1 antibody was detected by sandwich Enzyme-linked immunosorbent assay （ELISA）, and IgM of F1 antibody was determined with a capture antibody. Results After vaccinated Yersinia pestis for 3rd days, F1 antibody and IgM appeared slightly positive, with the titer of 1 ： 2 and 1 ： 20.33, respectively. The titer of F1 antibody increased to 1 ： 25.33 on the 4th day, and reached the peak of 1 ： 29.67 on the 97th day, kept 1 ： 25.33 on the 521st day. While the titer of IgM reached peak of 1 ： 212.0o on the 7th to lOth days, and decreased rapidly to below the positive standard on the 30th day. Conclusions Detection of F1 antibody in cats with plague by sandwich ELISA can trace plague prevalence in animals back to a long time, which may be applied for investigation of plague foci. A single serum sample can determine early animal plague with a capture antibody to detect IgM of F1 antibody in cat with the plague, and a single serum sample tested with the two methods at the same time for detection of F1 antibody and IgM can precisely verify the infection time of plague in animals for 3 to 7 days.

关 键 词：鼠疫 猫 F1抗体 免疫球蛋白M 

分 类 号：R516.8[医药卫生—内科学]