DNA条形码技术在黑线仓鼠分类研究中的应用  

An application of DNA barcoding in identification of Cricetulus Barabensis

作　　者：陈宝宝[1] 安翠红[2] 孙养信[1] 范锁平[1] 霍丽霞[1] 吕文[1] 佘建军[1] 

机构地区：[1]陕西省疾病预防控制中心消毒与病媒生物防控所,西安710054 [2]西安交通大学医学部,西安710054

出　　处：《中华地方病学杂志》2016年第5期325-328,共4页Chinese Journal of Endemiology

基　　金：陕西省科学技术研究发展计划（2012K16-12-03）

摘　　要：目的运用DNA条形码技术对陕西省境内黑线仓鼠标本进行种别鉴定。方法在陕西省陕北地区的定边、靖边县和关中地区的大荔县，采集65份黑线仓鼠标本（定边县58份、靖边县2份、大荔县5份），基于线粒体细胞色素C氧化酶I亚基（COI）基因进行序列分析，计算遗传距离，邻接法（NJ）构建系统发育树。结果定边县2份黑线仓鼠样本（13．16、13．21）与定边县其他56份样本的遗传距离为912％～10．0％，而56份样本与靖边县黑线仓鼠样本的遗传距离≤1％，与大荔县黑线仓鼠样本的遗传距离为7．2％～813％；靖边、大荔县内部样本的遗传距离≤l％。NJ系统树中，3个县黑线仓鼠样本分成两个高置信度的大分支，定边县2份样本（13．16、13-21）聚为一类，其余样本单独成支；定边县其余56份样本与靖边县样本在一个小分支上，大荔样本占据另一小分支。结论陕西省陕北、关中地区存在3种黑线仓鼠亚种，定边县有两种，大荔县为另一亚种。Objective To apply DNA barcoding technology for exploring its taxonomic status and differences in the molecular biology of Cricetulus barabensis in Shaanxi Province.Methods Sixty-five samples of Cricetulus barabensis were collected from Dingbian,Jingbian Counties in northern of Shaanxi and Dali County in Guanzhong plain (Dingbian 58 samples,Jingbian 2 samples,and Dali 5 samples).According to the mitochondrial cytochrome C oxidase subunit I gene (CO I) sequence,the genetic distance was calculated and Neighbor-Joining tree was constructed.Results The genetic distance between two samples (13.16,13.21) and other 56 samples of Dingbian was 9.2％-10.0％.The genetic distance between the 56 samples of Dingbian and Jingbian was less than 1％ and Dali was 7.2％-8.3％;the average intraspecific genetic distance of Jingbian and Dali was less than 1％.The Neighbor-Joining tree showed that all the Cricetulus barabensis samples from the three counties were separated into two large branches.The samples of 13.16,13.21 from Dingbian together were classified into a class and the rest of the samples into another separate branch.At the same time,other samples from Dingbian except 13.16,13.21 and Jingbian were distributed in a small branch,and Dali samples were occupied another small branch.Conclusion Using the DNA barcoding technology,we have determined three subspecies of Cricetulus barabensis in Shaanxi Province,Dingbian has two kinds and Dali has a different subspecies.

关 键 词：黑线仓鼠 DNA条形码 线粒体细胞色素氧化酶亚基I基因 

分 类 号：Q953[生物学—动物学]