抗稻瘟病基因Piz-t和Pi9连锁标记开发及在云南粳稻中的应用  

Development and Application of Molecular Markers Linked with Two Blast Resistance Gene Piz-t and Pi9 in japonica Cultivars of Yunnan

作　　者：刘树芳[1] 董丽英[1] 赵国珍[2] 李迅东[1] 杨勤忠[1] 

机构地区：[1]云南省农业科学院农业环境资源研究所,云南昆明650205 [2]云南省农业科学院粮食作物研究所,云南昆明650205

出　　处：《西南农业学报》2016年第4期721-725,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技厅对外科技合作计划(国际科技合作)项目(2014IA009);国家自然科学基金项目(31360462)

摘　　要：云粳优5号、云资粳41号和楚粳28号是农艺性状优良但对稻瘟病抗性弱的粳稻品种,采用回交的方法将2个广谱抗稻瘟病的基因Piz-t和Pi9导入这些品种以改良这3个品种的抗稻瘟病特性。为提高后代抗病材料选择的准确性和效率,本研究开发获得5个与Piz-t和Pi9基因紧密连锁并且在供体亲本和受体亲本间具有多态性的分子标记。利用分子标记跟踪检测来源于供体亲本与受体亲本回交获得的BC1F1单株中Piz-t和Pi9基因,并进一步利用对受体亲本云粳优5号、云资粳41号和楚粳28号致病,但对持有Piz-t和Pi9基因的供体亲本不致病的稻瘟病菌09BSH-10-5A进行接种分析。结果显示,携带有供体亲本和受体亲本DNA片段的杂合单株均表现抗病,而仅携带受体亲本DNA片段的个体均表现为感病。表明所开发的5个分子标记可用于抗病育种的分子标记辅助选择,有效提高抗病材料选拔的准确性与效率,创制新的抗源材料。Three japonica cultivars,Yunjingyou 5,Yunzijing 41 and Chujing 28 had elite agronomic traits such as high yield and good quality,but weak resistance to rice blast. Two genes Piz-t and Pi9 with broad-spectrum resistance to rice blast were introgressed into them to improve their resistance to rice blast by backcross strategy. In order to improve the accuracy and efficiency of selection of resistance individuals to rice blast,5 molecular markers polymorphic among parents,and closly linked with Piz-t and Pi9 genes,were developed. These markers were used for genotyping the BC1F1 plants derived from the crosses between susceptible japonica cultivars and resistant donors. The BC1F1 plants were further inoculated with Magnaporthe oryzae strain 09BSH-10-5A,which was virulent to Yunjingyou 5,Yunzijing 41 and Chujing28,but avirulent to Toride 1 and 75-1-127 carrying Piz-t and Pi9 gene,respectively. The result showed that heterozygous BC1F1 individuals carrying the DNA fragments of both parents were resistant to M. oryzae strain 09BSH-10-5A,but the individuals carrying only DNA fragment of receptors were susceptible to M. oryzae strain 09BSH-10-5A. These results indicated that five molecular markers developed in this study were suitable for marker-assisted selection in disease-resistant breeding,which could effectively improve the accuracy and efficiency of the selection of disease-resistant materials,and create new resistant resources.

关 键 词：稻瘟病 抗性基因 Piz-t Pi9 分子标记 

分 类 号：S511[农业科学—作物学]