葡萄糖在聚对苯二胺/铜修饰电极上的电化学行为及测定  

Electrochemical Behavior and Determination of Glucose at Poly(p-phenylenediamine)/Cu Modified Electrode

作　　者：张文丽[1] 李容[1] 常晓璇 苟兴龙[1] 

机构地区：[1]西华师范大学化学化工学院,四川南充637002

出　　处：《分析测试学报》2016年第5期508-513,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21571148);四川省教育厅重点资助项目(13ZA0014)

摘　　要：在静态法合成聚对苯二胺纳米片的基础上，经一步水热将铜微球均匀锚定于其上，成功合成了具有良好导电性、大比表面积、大孔径和孔容的铜／聚对苯二胺（Cu／PpPD）复合物，其独特的结构有利于电子的转移、活性位点的充分利用以及反应物、电解质等的输运。复合物对葡萄糖氧化表现出很高的电催化活性，在最优测试条件下，所构建的葡萄糖无酶传感器响应时间短（达到稳定电流的95％所需时间小于3s）、线性范围宽（0．003～6．44mmoL／L）、灵敏度高（929μA·mmol^-1·L·cm^-2）、检出限低（4．48×10^-7mol／L）、重现性和选择性好，对血清样品进行检测，回收率为99．5％～101．1％。所制备Cu／PpPD复合物能实现对葡萄糖的简单、快速、灵敏、准确无酶检测，在临床医学上糖尿病人的早期诊断和治疗监测领域具有很好的应用前景。Poly（p-phenylenediamine） nanosheets were first synthesized through oxidative polymeriza- tion of p-phenylenediamine, and then used as the substrate for anchoring copper microspheres via a facile solvothermal route. The resultant copper/poly （p-phenylenediamine） （Cu/PpPD） composites possess good electrical conductivity, big specific surface area, large pore size and pore volume, which are beneficial for transfer of electrons, utilization of active sites and transportation of reactant and electrolyte. The as-prepared Cu/PpPD composites showed excellent electrocatalytic activity to- ward the direct oxidation of glucose in the absence of any enzymes. Under the optimal conditions, the Cu/PpPD-based enzymeless glucose sensor showed the advantages of short response time （i. e. , the current achieved 95% of the steady state value within 3 s）, wide linear range （ 0. 003 - 6.44 mmol/L）, high sensitivity（929 μA · mmol^-1· L · cm^-2）, low detection limit（4.48 × 10^-7 mol ·L^-1 ）, good reproducibility and selectivity. In addition, the as-prepared Cu/PpPD-based enzymeless glucose sensor was successfully applied in the quantitative determination of glucose in real serum sam- ples with recoveries of 99.5% - 101.1% , which indicated it has a great prospect in clinical application.

关 键 词：聚对苯二胺 铜微球 葡萄糖 无酶传感器 

分 类 号：O657.1[理学—分析化学] TS245.4[理学—化学]