离子液体作流动相添加剂高效液相色谱法分离莨菪类生物碱  

Separation of Tropane Alkaloids by HPLC with Ionic Liquid as Mobile Phase Additive

作　　者：方海红[1,2] 朱益雷 魏惠珍[3] 金浩鑫[3] 殷文静[2] 饶毅[2] 

机构地区：[1]江西科技师范大学药学院,江西南昌330013 [2]江西中医药大学药学院,江西南昌330006 [3]中药固体制剂制造技术国家工程研究中心,江西南昌330006

出　　处：《分析测试学报》2016年第5期614-617,共4页Journal of Instrumental Analysis

基　　金：江西省科技厅青年科学基金项目(20132BAB215024);江西省研究生创新专项资金项目(YC2015-S348);江西中医药大学校级硕士研究生创新专项资金项目(JZYC15S2);江西省教育厅科研项目(GJJ13580);江西科技师范大学科研项目(2013XJZD004)

摘　　要：建立了以离子液体1-辛基-甲基咪唑六氟磷酸盐(Omin PF6)为高效液相色谱流动相添加剂分离莨菪类生物碱的方法,探讨了离子液体的保留模型及机理。采用高效液相色谱法,考察了检测波长、有机相种类和比例、离子液体的种类、p H值和浓度、缓冲盐体系等因素对莨菪类生物碱色谱行为的影响。结果显示,当离子液体作为流动相添加剂时,可明显改善此类生物碱的分离效果,减少色谱峰的拖尾,提高分离效率。研究显示,Omin PF6浓度与容量因子的变化符合溶质计量置换保留模型(SDM-R),且保留过程以竞争吸附为主。A novel analytical method was developed for the separation of tropane alkaloids by high performance liquid chromatography（HPLC） using ionic liquid （OminPF6 ） as mobile phase additive. The retention model and mechanism for the ionic liquid were also illustrated. The effect factors, in- cluding wavelength of detection, type and proportion of organic phase, pH value, type and concen- tration of ionic liquid and buffer salt system on retention behavior of components were investigated. The results showed that, the ionic liquid as mobile phase additive could significantly improve the sep- aration effect on tropane alkaloids, and also increase the symmetry of peak and the relative separa- tion. The results also showed that the relationship between capacity factor and concentrations of ionic liquid preferably complied with the stoiehiometric displacement model for retention （ SDM - R）, and the retention process of alkaloids was mainly based on competitive adsorption function.

关 键 词：离子液体 高效液相色谱法 莨菪类生物碱 色谱行为 保留机理 

分 类 号：O657.72[理学—分析化学] TQ460.72[理学—化学]