适配体电化学传感器检测汞离子进展  

Progress on Detection of Mercury Using Aptamer Electrochemical Sensor

作　　者：梁顺超 魏小平[1] 黄文刚[1] 李建平[1] 

机构地区：[1]广西岩溶地区水污染控制与用水安全保障协同创新中心,桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《分析测试学报》2016年第5期618-626,共9页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21375031);广西自然科学基金(2015GXNSFFA139005,2015GXNSFAA139029);广西高校食品安全与检测重点实验室项目

摘　　要：适配体是一小段经体外筛选得到的寡核苷酸序列。适配体中的胸腺嘧啶(T)碱基可与Hg^(2+)形成比双链DNA更加稳定的T-Hg^(2+)-T结构。利用该性质结合电化学测量方法可制作检测Hg^(2+)的特异性强、灵敏度高的适配体电化学传感器,并建立微量Hg^(2+)的检测方法。该文对近年来发展的检测Hg^(2+)的适配体电化学传感器进行了综述和总结,对文献报道的几类传感器的构建过程和检测机理进行了详述,对检测方法的优缺点进行了分析。最后,对此类传感器今后的发展方向提出了展望,引用文献83篇。Aptamer is a subparagraph ligonucleotide sequence screened in vitro, and has a high af- finity and strong specificity to combine with corresponding ligands. Thymine（T） bases can combine specially with Hg^2＋ to form the structure of T -Hg^2＋ -T, which is more stable than the double helix of DNA. Based on this structure, an aptamer electrochemical sensor was fabricated to detect Hg^2＋ , and it showed the advantages of high specificity, stability and sensitivity. In this paper, the devel- oped aptamer electrochemical sensors for Hg2~ detection in recent years are summarized and re- viewed. The fabrication processes and the detection mechanisms of this kind of sensors are reported in detail, the merit and demerit of the aptamer electrochemical sensor are analyzed. Finally, the develo- poing prospect for the sensors in the future are put forward, and 83 references are cited.

关 键 词：适配体 HG^2+ 电化学传感器 综述 

分 类 号：O657.1[理学—分析化学] O614.243[理学—化学]