有机双光子线粒体内活性小分子荧光探针研究进展  

Research Progress in Organic Two-photon Fluorescent Probes for Active Small Molecules in Mitochondria

作　　者：杨志广[1] 李运林[1] 陈亚红[1] 郭雷[1] 张建夫[1] 彭鹏[1] 

机构地区：[1]周口师范学院化学化工学院,河南周口466001

出　　处：《分析测试学报》2016年第5期627-634,共8页Journal of Instrumental Analysis

基　　金：河南省高等学校重点科研项目计划资助(15A430055);周口师范学院校本项目资助(ZKNUB115201)

摘　　要：线粒体在细胞的能量代谢中发挥着关键作用,其内部环境的微小变化会影响细胞的正常生命活动。同时线粒体内许多活性小分子在细胞的许多生理过程中也起着关键作用。因此,可视化监测线粒体自身及内部微环境的变化对于生命现象的研究和疾病的诊断与治疗具有非常重要的意义。荧光分析法因具有操作简便、灵敏度高、选择性好、实时检测以及损伤小等优点而得到了广泛的研究和应用。双光子荧光探针技术相对于单光子荧光技术具有长波吸收短波发射、高度的三维空间选择性、大的穿透深度、避免荧光漂白和光致毒以及降低组织自发荧光干扰等特点,在生命科学领域具有广阔的应用前景。该文介绍了有机双光子吸收基本原理以及有机双光子线粒体内活性小分子荧光探针的研究现状,同时对有机线粒体内活性小分子荧光探针今后的发展趋势进行了展望。Mitochondria plays a key role in energy metabolism of ceils, and the small changes of in- ternal environment in mitochondria will affect the normal cell life activities. In the meanwhile, many active small molecules in mitochondria also play a key role in many physiological process. Therefore, visual monitoring of mitochondrial itself and its changes of the internal micro environment are of great importance for the research of biological phenomenon, disease diagnosis and treatment. Fluorescence method has been extensively used due to its advantages of simple operation, high sensitivity and se- lectivity, real-time detection and little damage to organisms. The two-photon fluorescent probe tech- nique has shown a much broader application prospect in the field of life sciences compared to the one- photon fluorescent probe technique as it has many advantages, such as absorbing light of longer wave- length with lower energy but emitting shorter wavelength, high three-dimensional spatial selectivity, large penetration depth, avoidance of photodamage and photobleaching and lower tissue auto-fluorescence. The basic principle of organic two-photon absorption and the research status of organic two-photon fluores- cent probes for active small molecules in mitochondria were introduced, as well as the trends of organic fluorescent probes for active small molecules in mitochondria in the future were prospected.

关 键 词：线粒体 活性小分子 双光子 荧光探针 检测 综述 

分 类 号：O622[理学—有机化学] TP212.2[理学—化学]