毛细管电泳电化学发光检测香草扁桃酸和高香草酸  

Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection

作　　者：杨洋[1] 孙雪梅[1] 李传龙[1] 

机构地区：[1]青岛科技大学化学与分子工程学院生态化工教育部重点实验室,山东青岛266042

出　　处：《河北大学学报（自然科学版）》2016年第3期257-263,共7页Journal of Hebei University(Natural Science Edition)

基　　金：山东省自然科学基金资助项目(ZR2009BM029)

摘　　要：利用毛细管电泳电化学发光(CE-ECL)法检测了尿样中的香草扁桃酸(VMA)和高香草酸(HVA),得到了最佳的分离检测条件:50 mmol/L磷酸盐缓冲溶液(pH 8.2);分离电压16kV;检测电势1.2V(相对于饱和甘汞电极).在最佳条件下,VMA和HVA的浓度检测限(S/N=3)分别为5.0×10-7 mol/L和6.1×10-7 mol/L,线性回归系数分别为0.999 3和0.997 9.并以此方法将尿液中的VMA和HVA在10min内有效地分离检测,不受其他干扰,得到加标回收率分别为98%和99%,取得了满意的结果.Vanilla mandelic acid（VMA）and homovanillic acid（HVA）in the urine were detected by capillary electrophoresis with electrochemiluminescence detection（CE-ECL）.The following conditions were suitable for the determination of VMA and HVA：running buffer,50mmol/L NaH2PO4-Na2HPO4（pH=8.2）;separation voltage,16kV;detection potential,1.2V（vs.saturated calomel electrode（SCE））.Under the optimum conditions of detection,satisfactory sensitivities（LOD of 5.0×10^（-7） mol/L for VMA and 6.1×10^（-7） mol/L for HVA at S/N=3）were obtained,the correlation coefficients of the method were 0.999 3and 0.997 9,respectively.The determination of these metabolites in human urine was completed within10 min without any interferences,the recoveries were VMA 98%and HVA 99%,the result obtained is satisfactory.

关 键 词：毛细管电泳 电化学发光检测 香草扁桃酸 高香草酸 

分 类 号：O657.8[理学—分析化学]