单核细胞趋化蛋白-1A-2518G基因多态性与2型糖尿病及葡萄糖耐量异常的关系  

Relationship of polymorphism of monocyte chemoattractant protein-1A-2518G gene with type 2 diabetes mellitus and impaired glucose tolerance

作　　者：陈冰[1] 王美宁[1] 何海宁 黄勇奇[3] 蒋杰球[4] 唐智清 刘巧玲[1] 

机构地区：[1]广西医科大学附属南宁市第一人民医院内科,南宁市530022 [2]南方电网公司医务所 [3]广西医科大学生化教研室 [4]广西医科大学第一附属医院检验科,南宁市530022

出　　处：《广西医学》2016年第4期471-474,478,共5页Guangxi Medical Journal

基　　金：广西自然科学基金(桂科自0640198)

摘　　要：目的探讨单核细胞趋化蛋白-1(MCP-1)A-2518G基因多态性与葡萄糖耐量异常(IGT)、新发2型糖尿病(T2DM)的关系。方法对111例健康人(NC组)、82例IGT患者(IGT组)及76例新发T2DM患者(T2DM组)采用聚合酶链反应-限制性片段长度多态性技术,观察MCP-1A-2518G基因多态性,分析其与IGT和T2DM的关系。结果 IGT、T2DM组与NC组的MCP-1等位基因和基因频率比较差异无统计学意义(P>0.05),logistic回归分析结果显示,腰臀比、胰岛素抵抗指数、MCP-1 AA是IGT及新发T2DM发病的独立危险因素(P<0.05)。结论 MCP-1 A-2518G基因多态性可能参与IGT及新发T2DM的发病机制,AA基因携带增加人群IGT及新发T2DM的患病风险。Objective To explore the relationship of polymorphism of monocyte chemoattractant protein-1 （MCP-1）A-2518G gene with impaired glucose tolerance （IGT） and new-onset type 2 diabetes mellitus （T2DM）. Methods A total of 111 healthy individual （NC group） ,82 patients with IGT（IGT group） and 76 patients with new-onset T2DM（T2DM group） were enrolled. The polymorphism of MCP-1 A-2518G gene was observed using a polymorphism chain reaction（PCR） and restriction fragment length polymorphism technology, and its relationship with IGT and T2DM was analyzed. Results There were no significant differences in alleles or genotype frequencies of MCP-1 gene among NC group, IGT group and T2DM group（P 〉 0.05）. The result of logistic regression analysis showed that waist/hip ratio, insulin resistance index and MCP-1 AA were the independent risk factors for IGT and new-onset T2DM（P 〈 0.05）. Conclusion MCP-1 A-2518G polymorphism may be involved in the pathogenesis of IGT and new-onset T2DM. AA gene carriers may increase the morbidity risk of IGT and new-onset T2DM.

关 键 词：2型糖尿病 葡萄糖耐量异常 单核细胞趋化蛋白-1 胰岛素抵抗 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]