超声心动图引导建立小型猪主动脉瓣反流模型的方法学研究  

Establishment of Aortic Regurgitation Model in Chinese Miniature Pigs Under Echocardiography Guidance

作　　者：孙妍[1] 王建德[1] 李晓妮[1] 张丽[1] 张茗卉[1] 唐越[2] 贾六军[2] 孟亮[2] 王浩[1] 

机构地区：[1]中国医学科学院,北京协和医学院,国家心血管病中心,阜外医院超声科,北京市100037 [2]中国医学科学院,北京协和医学院,国家心血管病中心,阜外医院动物实验中心,北京市100037

出　　处：《中国循环杂志》2016年第5期495-498,共4页Chinese Circulation Journal

基　　金：国家自然基金项目(81271583)

摘　　要：目的:研究如何应用超声心动图引导建立小型猪主动脉瓣反流模型。方法:选用中华小型猪,静脉全麻后测量体重,并行超声心动图检查,排除主动脉瓣病变。将实验猪颈部小切口,暴露颈动脉,置入鞘管后送导管至主动脉窦部,用硬导丝穿出导管,调整导管位置,造成主动脉瓣损伤。超声评估主动脉瓣损伤情况及反流量。处死动物,取心脏,观察主动脉瓣损害情况。结果:共入选中华小型猪7只,雄性4只,雌性3只,动物平均体重(24.7±3.6)kg。共5只顺利完成超声引导下主动脉瓣反流模型,造成少量反流1只,少-中量反流1只,中量反流2只,大量反流1只。其中4只造成瓣叶穿孔,1只造成瓣叶撕裂。结论(:1)超声心动图可以顺利引导导丝穿过主动脉瓣并导致不同程度主动脉瓣损伤,用于小型猪主动脉瓣反流造模方法可靠。(2)超声心动图可明确主动脉瓣损伤部位及损伤程度。Objective： To establish the aortic regurgitation model in Chinese miniature pigs under echocardiography guidance. Methods： The animal models were established by following steps： general anesthesia, measuring body weight and then receiving echocardiography examination to exclude aortic valve lesions; carotid artery was exposured by surgery, catheter was sent to aortic sinus with stiff guide wire penetrates and the position of catheter was adjusted to obtain aortic valve damage. The aortic valve injury and regurgitation were evaluated by ultrasound; then the pigs were killed and the heart was taken to observe aortic valve damage. Results： A total of 7 pigs were used including 4 male and 3 female with the mean body weight of（24.7 ± 3.6） kg. Aortic regurgitation model was successfully established in 5 pigs including 1 mild, 1 mild-moderate, 2 moderate, 1 severe aortic valve regurgitation, and 4 were with valve leaflets perforation and 1 with leaflets tearing.Conclusion：① Echocardiography can smoothly guide wire go through aortic valve and make valve damage at different degrees, it is reliable to establish aortic valve regurgitation model in experimental pigs.② Echocardiography may clearly identify the position and degree for aortic valve injury.

关 键 词：模型 动物 超声心动描记术 主动脉瓣反流 

分 类 号：R541[医药卫生—心血管疾病]