先天性心脏病大动脉转位小鼠模型的建立  

Establishment of Congenital Heart Disease Transposition of Great Arteries in Experimental Mice Model

作　　者：高仕君 聂宇[1] 廉虹[1] 胡盛寿[1] 

机构地区：[1]北京协和医学院中国医学科学院国家心血管病中心阜外医院心血管疾病国家重点实验室,北京市100037

出　　处：《中国循环杂志》2016年第5期499-501,共3页Chinese Circulation Journal

基　　金：国家自然科学基金项目(81441010;81430006;81500239)

摘　　要：目的：尝试建立一种先天性心脏病大动脉转位小鼠模型,为探讨该类疾病发生、发展的相关机制提供条件。方法：20只8~10周大的SPF级ICR孕鼠随机分为对照组（n=5）和实验组（n=15）。实验组孕鼠在E8.5天给予单次剂量全反式视黄酸（70 mg/kg）腹腔注射,对照组孕鼠在E8.5天给予单次剂量二甲基亚砜（70 mg/kg）腹腔注射。注射完毕后继续饲养至E18天后取出胚胎,在体式显微镜下观察心脏表型。结果：与对照组相比,实验组胚胎的早产、流产、胚胎吸收的发生率显著增加;大动脉转位表型明显,发生率为61.2%,同时合并突眼、神经管畸形等多种心外表型发生。结论：全反式视黄酸可以诱导小鼠胚胎发生大动脉转位,是一种可行的疾病动物模型。Objective： To establish a mice model of congenital heart disease transposition of great arteries in order to provide a research reference for the occurrence and development of transposition of great arteries.Methods： A total of 20 pregnant ICR mice at 8-10 weeks of age were divided into 2 groups： Control group, the mice received a single dose of DMSO 70 mg/kg at 8.5 days of gestation, n=5 and Experiment group, the mice received a single dose of all-trans retinoic acid 70 mg/kg at 8.5 days of gestation, n=15. All animals were treated for 18 days and then the embryos were taken to observe cardiac morphology under stereomicroscope.Results： Compared with Control group, Experiment group had obviously increased occurrence rates of premature delivery, abortion and embryo absorption, and 61.2% phenotype for transposition of great arteries; meanwhile, combining with non-heart defect phenotypeas exophthalmos and spinal malformation. Conclusion： All-trans retinoic acid may induce transposition of great arteries in mice embryos, which is a feasible animal model in experimental research.

关 键 词：心脏缺损 先天性 大血管错位 全反式视黄酸 模型 动物 

分 类 号：R54[医药卫生—心血管疾病]