不同浓度的艾烟对昆明小鼠的急性毒性研究  

Acute toxicity study on different concentrations of moxa smoke in KM mice

作　　者：韩丽[1,2] 胡海[2,3] 刘平[2,4] 蔡虹[2,5] 杨佳[2] 朱茂祥[6] 杨陟华[6] 潘秀颉[6] 黄畅[2] 刘耀萌 哈略 邬继红[2] 赵百孝[2] 

机构地区：[1]北京中医药大学中医学院,北京100029 [2]北京中医药大学针灸推拿学院,北京100029 [3]北京中医药大学附属护国寺中医医院,北京100035 [4]华北电网有限公司北京电力医院,北京100073 [5]重庆渝北区中医院,重庆401120 [6]中国人民解放军军事医学科学院,北京100039

出　　处：《中华中医药杂志》2017年第2期731-734,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81403449;No.81373730);国家重点基础研究发展计划(973计划)项目(No.2009CB522906)~~

摘　　要：目的:观察艾烟对昆明小鼠(KM)的急性毒性。方法:选用采用全身整体暴露的方法,应用全自动动式染毒柜CSED-A,将3组KM小鼠(n=36,雌雄各半),分别暴露于遮光率为85%、65%、50%的艾烟中,记录各组小鼠的毒性反应以及最终艾烟暴露时间,进而回归分析得出各组半数致死时间(LT50)。结果:小鼠表现为呼吸困难,多泪和明显增多的无色的鼻腔分泌物,运动失调,强直性抽搐和角弓反张。85%、65%、50%艾烟对雄鼠的LT50(38.59、283.05、315.87min)低于雌性(51.62、298.46、386.99min);全体的85%、65%、50%艾烟的LT50分别为45.77、295.33、303.94min。结论:超高浓度的艾烟在足够长的暴露时间导致小鼠死亡,但不支持临床接触艾烟即存在安全隐患,本实验为后期艾烟安全剂量和有效剂量的研究提供参考。Objective:To investigate the acute toxicity of moxa smoke in KM mice.Methods:The whole body overall exposure method was applied.A dynamic smoke exposure device(CSED-A) was used.3 sets(n=36, male∶female=1∶1) of KM mice were exposed to moxa smoke of 85%, 65%, and 50% of shading rate.Exposure duration and toxic reaction of all the mice were recorded to calculate the acute median lethal time in different groups.Results:Clinical signs of toxicity such as difficult breathing, eye and nasal discharge, ataxia and tonic convulsion and opisthotonos before dying were observed during exposure period.In each group, the LT50 for male mice(38.59 min, 283.05 min and 315.87min) were lower than the female mice(51.62 min, 298.46 min, 386.99min) with moxa smoke of 85%, 65%, and 50%.The LT50 at the moxa smoke of 85%, 65%, and 50% were 45.77 min, 295.33 min, and 303.94 min, respectively.Conclusion:Moxa smoke at high concentration over an estimated ‘worst case' scenario for exposure caused death in mice.This does not, however, mean that the moxibustion environment is unsafe in clinic.This study provides reference for future studies on the safe dose and effective dose of moxa smoke.

关 键 词：艾灸 艾烟 毒性 暴露 安全性 

分 类 号：R285.5[医药卫生—中药学]