平喘颗粒质量标准研究  

Study on quality standard of Pingchuan Granules

作　　者：蒋鹏娜[1] 李星[1] 王雪慧[1] 李竹英[1,2] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室,哈尔滨150040

出　　处：《中华中医药杂志》2017年第5期2132-2136,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学青年基金项目(No.8140323);黑龙江省自然科学基金项目(No.H201481);黑龙江中医药大学"优秀创新人才支持计划"项目(No.051268);黑龙江中医药大学新药研究基金项目(No.2014xy04)~~

摘　　要：目的:建立平喘颗粒的质量标准。方法:采用薄层色谱法(TLC)法对平喘颗粒中淫羊藿、黄芪、炙麻黄、五味子进行定性鉴别,并用高效液相色谱法(HPLC)测定平喘颗粒中淫羊藿苷、黄芪甲苷的含量。结果:TLC能定性检出淫羊藿、黄芪、炙麻黄、五味子,斑点清晰,分离度好,且阴性对照无干扰;淫羊藿苷、黄芪甲苷分别在7.6-76.0μg/mL(r=0.9998)、0.99-31.85μg/mL(r=0.9994)范围内与峰面积均呈现良好的线性关系,平均回收率分别为96.50%、99.53%。结论:所建立的定性、定量检测方法操作简便,专属性强、重复性好、结果准确可靠,可作为平喘颗粒的质量控制方法。Objective: To establish the quality standard for Pingchuan Granules. Methods: TLC method was used for qualitative identification of Herba Epimedii, Radix Astragali seu Hedysari, honey-fried Herba Ephedrae, and Fructus Schisandrae Chinensis. HPLC method was used to determine the contents of Icariin and Astragaloside Ⅳ in the granules. Results: The TLC method can qualitatively identify the Herba Epimedii, Radix Astragali seu Hedysari, honey-fried Herba Ephedrae, and Fructus Schisandrae Chinensis. The spots were clear with good separation and without interference in the negative reference. The Icariin and Astragaloside Ⅳ showed a good linearity in the measurement range of 7.6-76.0μg/mL(r=0.9998), 0.99/31.85μg/mL(r=0.9994). The average recoveries were 96.50% and 99.53%, respectively. Conclusion: The established qualitative and quantitative detection method is simple, specific, reproducible, accurate and reliable and can be used for quality control of Pingchuan Granules.

关 键 词：平喘颗粒 淫羊藿苷 黄芪甲苷 薄层色谱 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学]