通络保肾复方对糖尿病大鼠肾脏内质网应激介导的凋亡信号通路JNK、Caspase-12的影响  

Effects of Tongluo Baoshen Formula on apoptosis signaling pathways JNK,Caspase-12 mediated by endoplasmic reticulum stress

作　　者：张杰 荣小龙 李建民 刘秀萍 周国民 黄彩霞 李素霞 张晓倩 

机构地区：[1]北京市中西医结合医院肾病科,北京100039

出　　处：《中华中医药杂志》2017年第11期5109-5112,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81273706)~~

摘　　要：目的:探讨通络保肾复方及其拆方对糖尿病大鼠肾损伤内质网应激凋亡通路的影响。方法:将SD大鼠随机分成正常组、造模组,腹腔注射STZ建造糖尿病大鼠模型,造模成功后随机分成模型组、缬沙坦组、通络保肾复方组、中药通络保肾补虚组、中药通络保肾解毒组。喂养6周后测大鼠血糖(GLU)、糖化血红蛋白(Hb A1c)、血浆白蛋白(ALB)、血肌酐(Scr)及肾组织GRP78、p-JNK、Caspase-12蛋白及基因的表达。结果:6周时与正常组比较,模型组GLU、HbA1c、BUN升高(P<0.01);与模型组比较,缬沙坦组GLU、HbA1c、BUN下降(P<0.01);各中药治疗组HbA1c下降(P<0.01)。Western blot、qRT-PCR结果:与正常组比较,模型组大鼠GRP78、p-JNK表达上调(P<0.01);与模型组比较,各治疗组表达下调(P<0.01,P<0.05)。结论:通络保肾复方及其拆方可抑制内质网应激诱导JNK凋亡通路激活,6周时无Caspase-12信号激活。结合中医学方证理论,以方测证,推测糖尿病肾损伤的中医核心病机为本虚标实。Objective: To discuss the influence of Tongluo Baoshen Formula and its disassembled formula on renal injury ERS apoptosis signaling pathways in diabetic rats. Methods: SD rats were randomly divided into normal group and model group. The diabetic rat model was established by intraperitoneal injection of STZ, then the model rats were randomly divided into model group, valsartan group, Tongluo Baoshen Formula group, Tongluo Baoshen Buxu group and Tongluo Baoshen Jiedu group. The blood glucose(GLU), HbA1c, ALB, Scr and the expression of GRP78, p-JNK, Caspase-12 were detected after 6 weeks. Results: Compared with control group, the GLU, HbA1c and BUN in model group increased significantly in 6 th week(P<0.01). Compared with model group, the GLU, Hb A1 c and BUN in valsartan group were obviously decreased(P<0.01), the HbA1c in the traditional Chinese medicine treatment group was obviously decreased(P<0.01). Western blot and q RT-PCR results: The expression of GRP78, p-JNK in model group increased compared with control group(P<0.01). Compared with model group, the expression of GRP78, p-JNK in all treatment groups decreased(P<0.01, P<0.05). Conclusion: Tongluo Baoshen Formula and its disassembled formula could inhibit JNK apoptosis signaling pathway induced by ERS, and there are still no obvious Caspase-12 apoptosis signal activation at week 6. Combined with TCM theory, which speculated that traditional Chinese medicine central pathogenesis of diabetic kidney injury is deficiency manifestation and excessive root cause.

关 键 词：内质网应激 细胞凋亡 糖尿病 通络保肾复方 JNK CASPASE-12 本虚标实 信号通络 

分 类 号：R285.5[医药卫生—中药学]