转基因大豆豆粕外源基因和蛋白在SD大鼠体内消化吸收分析  

Analysis of exogenous gene and protein digestion and absorption of SD rats(Rattus norvegicus) fed roundup ready soybean meal

作　　者：袁建琴[1] 常泓[1] 赵江河[1] 史宗勇[1] 王俊东[2] 

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《生物工程学报》2016年第5期657-668,共12页Chinese Journal of Biotechnology

基　　金："十二五"国家科技支撑计划(No.2012BAD12B06-2);山西省科技攻关项目(No.20140311025-3);山西省自然科学基金(No.2013011028-2);山西省高等学校教学改革项目(No.J2012026)资助~~

摘　　要：为了评估转基因抗草甘膦除草剂大豆的食用安全性,以20%的比例将转基因抗草甘膦除草剂大豆GTS40-3-2和其亲本非转基因大豆A5403豆粕分别添加到基础饲料中喂养两代Sprague-Dawley(SD)大鼠,采用定性、定量PCR和ELISA方法检测转基因大豆成分相关基因和蛋白在长期饲喂的大鼠体内代谢残留状况。结果表明,大鼠喂养转基因大豆豆粕后,除了大鼠肠粪和盲肠内容物检测到有转基因成分的残留,肠道菌群和实质脏器均未发现相关基因和蛋白。结果提示,长期饲喂转基因抗草甘膦除草剂大豆GTS40-3-2与亲本A5403大豆豆粕对SD大鼠具有同样的食用安全性。Metabolism and deposition of exogenous gene and protein from transgenic glyphosate herbicide-tolerant soybean meal in SD rats were studied in the experiment. The transgenic soybean GTS40-3-2 meal and its non-transgenic counterpart（parent A5403） were fed to the generation and the second generation Sprague-Dawley（SD） rats（Rattus norvegicus）. The study added the genetically modified（GM） soybean meal and its non-transgenic control soybean meal（parent A5403） in a ratio of 20% respectively to the feeds. By using qualitative, quantitative PCR and ELISA methods to detect transgenic soybean residues of metabolism ingredients in rats, the safety and influence of GM soybean were evaluated. The results showed that the intestinal fecal and cecum contents of rats were detected with residues of GM ingredients, intestinal flora and organs were not found related genes and protein. These results indicated that transgenic glyphosate herbicide-tolerant soybean GTS40-3-2 meal was as safe as its non-GM soybean meal in long-term feeding study.

关 键 词：抗草甘膦除草剂 转基因大豆GTS40-3-2 SD大鼠 外源基因和蛋白 消化吸收 

分 类 号：S565.1[农业科学—作物学]