薄皮甜瓜果实早期发育的细胞学观察及内源激素变化  

Cytological observation and changes of endogenous hormones in melon fruit during early development

作　　者：吴贝[1] 季玉洁[1] 李盼盼[1] 范双喜[1] 郝敬虹[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《北京农学院学报》2016年第2期64-68,共5页Journal of Beijing University of Agriculture

基　　金：国家自然科学基金委项目"薄皮甜瓜果实发育早期细胞膨大相关蛋白的鉴定及其相互作用分析"(31201641);北京市科学技术委员会科技新星项目"生长素响应因子ARF在调控甜瓜座果中的作用及其机理研究"(Z131105000413);北京市教育委员会项目"科研基地建设-科技创新平台-蔬菜创新平台建设"(PXM2014_014207_000028);北京市教育委员会项目"科研基地建设-植物生产类研究生种业实验实习平台建设"(PXM2014_014207_000073);北京市教育委员会蔬菜协同创新项目;国家科技部十二五科技支撑项目"京郊农业产业循环技术集成与示范:蔬菜废弃物养分资源循环技术"(2012BAD14B09)

摘　　要：【目的】分析不同授粉方式对薄皮甜瓜内源激素变化的影响,揭示薄皮甜瓜果实早期发育的规律。【方法】以薄皮甜瓜(Cucumis melo L.)"京蜜11号"为试材,设定坐果剂蘸花授粉和人工授粉两种授粉方式为处理,以未授粉果实为对照,采用石蜡切片技术观察了薄皮甜瓜果实早期发育阶段的细胞学变化,并利用酶联免疫法测定了其发育过程中的内源激素含量变化。【结果】薄皮甜瓜开花后,人工授粉和坐果剂蘸花处理后果实逐渐大于未授粉果实,且开花3 d后差异非常明显。薄皮甜瓜生长前期,坐果剂蘸花和人工授粉后果实中生长素含量高于未授粉果实,赤霉素、细胞分裂素变化趋势与生长素相似,脱落酸含量显著低于未授粉果实。【结论】整体来看,薄皮甜瓜开花后3 d可能是细胞快速膨大的启动时期,内源激素含量的变化与果实前期发育关系密切。【Objective】To analyze the influence on the change of endogenous hormones under different pollination methods,and reveal the regulation in the early development of melon.【Methods】This study adopted melon（ Cucumis melo L.） ＂Jingmi 11＂as the test materials,based on different pollination methods of dipping flower pollination and artificial pollination,the non-pollination was control,observing cytological changes by technique of paraffin section before and after pollination. The content of hormone was determined by the method of enzyme linked immunosorbent assay during the early development of melon.【Results】Fruits with dipping flower pollination and artificial pollination were bigger than non-pollination treatment after flowing,and the phenomenon was more obvious after flowing for 3 d. The content of IAA with the treatments of dipping flower pollination and artificial pollination was bigger than non-pollination in the early development of melon. the changes of GA and ZR were similar with IAA. The contents of ABA with dipping flower pollination and artificial pollination were obviously lower than the fruits without pollination.【Conclusion】Integrated as a whole,the 3 d may be the starting period of cell expansion after flowering,the changes of endogenous hormones were closely relation to the growth of melon fruitduring early development.

关 键 词：薄皮甜瓜 果实早期发育 细胞形态 内源激素 

分 类 号：S642.9[农业科学—蔬菜学]