金铁锁离体快繁技术研究  

The Research on in Vitro Rapid Propagation of Psammosilene tunicoides

作　　者：李斌[1,2] 唐军荣[1,2] 陈杰 尹丽莎 韩国伟[1,2] 于鑫 辛培尧[1,2] 

机构地区：[1]西南林业大学云南省高校林木遗传改良与繁育重点实验室,云南昆明650224 [2]西南林业大学林学院,云南昆明650224 [3]毕节市中药研究所,贵州毕节551700 [4]武警昆明市森林支队,云南昆明650118

出　　处：《西南林业大学学报（自然科学）》2016年第3期80-85,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：西南林业大学云南省省级重点学科(林学)资助;云南省省院省校教育合作咨询共建重点学科项目(No.211015)资助;西南地区生物多样性保育国家林业局重点实验室开放基金资助

摘　　要：为了更有效保护金铁锁野生资源,以其嫩茎为外植体进行快繁技术的研究。结果表明:消毒方法为清水冲洗后用75%乙醇消毒15 s,0.1%升汞消毒10 min,污染率降低至3.61%。分化培养基为MS+2.0 mg/L 6-BA+0.05 mg/L TDZ+0.05 mg/L NAA,平均分化芽数为3.49;增殖培养基为MS+0.3 mg/L 6-BA+0.05 mg/L TDZ+0.01 mg/L NAA,增殖倍数达4.29;生根培养基为1/2MS+0.3 mg/L IBA+0.1 mg/L NAA+0.3 g/L活性炭,生根率为91.7%;组培苗转入红土∶腐殖土∶珍珠岩=1∶1∶1的基质中成活率可达94.5%。该组织培养快繁技术,对解决其野生资源贫乏及生产上原材料供应不足的问题具有重要意义。In order to protect the wild resource of Psammosilene tunicoides effectively, tender stems of P. tunicoides were used as explants to study the in vitro rapid propagation technique of P. tunicoides. The results showed that the method of sterilization was that tender stems were rinsed with water, then disinfect with 75% alcohol for 15 s and 0. 1% mercuric for 10 min. The contamination rate was 3.61%. Differentiation culture medium was MS ＋ 2.0 mg/L 6-BA ＋ 0.05 mg/L TDZ ＋ 0. 05 mg/L NAA, the average quantity of differentiation bud was 3.49, proliferation culture medium was MS ＋ 0. 3 mg/L 6-BA ＋ 0.05 mg/L TDZ ＋ 0.01 mg/L NAA, the proliferation multiple was 4.29, Rooting culture medium was 1/2MS ＋ 0. 3 mg/L IBA ＋ 0. 1 mg/L NAA ＋ 0. 3 g/L activated carbon, the rooting rate was 91.7%, the tissue culture seedlings were transplanted into the matrix （red soil ： humus ： perlite=1 ： 1 ： 1 ）, the survival rate was 94.5%. The technology of tissue culture is hopeful to solve the problem of wild resource shortage and insufficient supply of raw materials in the production.

关 键 词：金铁锁 离体快繁 外植体 炼苗 培养基 

分 类 号：S723.1[农业科学—林木遗传育种]