氟伐他汀对人脐静脉内皮细胞的保护作用  

Protective effect of fluvastatin on human umbilical vein endothelial cells

作　　者：刘芳[1] 丁翊[1] 周春蕾[2] 

机构地区：[1]解放军第四五四医院干部病房,江苏南京210002 [2]解放军第四五四医院航空医学科,江苏南京210002

出　　处：《中国临床研究》2016年第5期675-678,共4页Chinese Journal of Clinical Research

基　　金：南京军区医学科技创新课题(12MA045)

摘　　要：目的研究氟伐他汀(Flu)对醛固酮环境下人脐静脉内皮细胞(HUVEC)增殖、凋亡的影响及其可能的机制。方法体外培养HUVEC细胞,分别以25、50、100 nmmol/L醛固酮干预HUVEC细胞24 h,细胞计数试剂盒(CCK^(-8))测定细胞增殖活力。以100 nmmol/L醛固酮干预细胞6、12、24 h,CCK^(-8)测定细胞增殖活力。100 nmmol/L醛固酮加不同浓度Flu(10^(-8)、10^(-7)、10^(-6)mmol/L)干预细胞24 h,CCK^(-8)测定细胞增殖活力。在此基础上,100 nmmol/L醛固酮处理细胞24 h,以不同浓度Flu(10^(-7)、10^(-8)、10^(-6)mmol/L)及加用细胞外信号调节激酶通路抑制剂PD98059(ERK1/2抑制剂)干预细胞,CCK^(-8)测定细胞增殖、流式细胞术测定细胞凋亡。结果醛固酮可抑制内皮细胞的增殖活力,并呈浓度依赖性和时间依赖性。100 nmmol/L醛固酮干预细胞24 h可明显抑制细胞增殖、诱导细胞凋亡。10^(-6)mmol/L Flu可促进醛固酮环境下细胞增殖、抑制细胞凋亡,并且其作用可被细胞外信号调节激酶通路抑制剂PD98059阻断。结论醛固酮可以抑制人脐静脉内皮细胞的增殖、促进其凋亡,Flu可减轻醛固酮抑制细胞增殖、诱导细胞凋亡的作用,其作用可被ERK/Z抑制剂阻断。Objective To investigate the effect of fluvastatin（ Flu） on the proliferation and apoptosis of human umbilical vein endothelial cells（ HUVECs） treated by aldosterone and its potential mechanism. Methods HUVECs incubated in vitro were respectively treated by 25,50,100 nmmol/L of aldosterone for 24 hours respectively,or by 100 nmmol/L of aldosterone for 6,12,24 h respectively,or by 10^（-8）,10^（-7）,10^（-6）mmol/L of Flu after treatment with 100 nmmol/L of aldosterone; after being treated by 100 nmmol/L of aldosterone for 24 hours followed by 10^（-6）mmol/L of Flu,the extracellular signal regulated kinase pathway 1/2（ ERK1/2） inhibitor-PD98059 was added to intervene. The cell counting kit^（-8）（ CCK^（-8））and flowcytometry were used to respectively detect the cell proliferation and cell apoptosis. Results Aldosterone can inhibit the proliferation activity of HUVECs with concentration-dependent manner and time-dependent manner. Compared with pre-treatment,after being treated with 100 nmmol/L of aldosterone for 24 hours,the cell proliferation was significantly inhibited,and cell apoptosis significantly increased,however,10^（-6）mmol/L of Flu can promote cell proliferation and inhibit cell apoptosis,but the effect of Flu can be antagonized by ERK1/2 inhibitor-PD98059. Conclusion Aldosterone can inhibit HUVECs proliferation and promote HUVECs apoptosis,while Flu can decrease the effects caused by aldosterone,but the effects of Flu can be antagonized by PD98059.

关 键 词：醛固酮 人脐静脉内皮细胞 氟伐他汀 增殖 凋亡 

分 类 号：R33[医药卫生—人体生理学]