贞蓉丹合剂对小鼠更年期综合征的影响  

Effects of Zhen Rong Dan Mixture on symptoms of menopausal syndrome in mice

作　　者：陈波[1] 张璇[1] 刘涛[1] 杨晓洪[1] 王芳芹 陈晓红[1] 

机构地区：[1]第三军医大学药学系药理教研室,重庆400038

出　　处：《中国临床药理学与治疗学》2016年第3期287-292,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：重庆市科技攻关项目(CSTC;2010AC5021)

摘　　要：目的:采用雌性老龄KM小鼠作为更年期综合征(menopausal syndrome,MP)动物模型,探讨贞蓉丹合剂(ZRD)对更年期综合征的治疗作用。方法:选取经阴道涂片法鉴定为更年期的KM小鼠作为MP小鼠,考察ZRD对MP小鼠自主活动、握力、戊巴比妥钠诱导睡眠及阈下剂量催眠、毛果芸香碱发汗实验、脏器指数的影响。结果:MP小鼠与青年小鼠相比,自主活动次数、握力时间、睡眠时间、胸腺系数与脾系数均显著减少,汗点数与脂肪系数显著升高;ZRD中、高剂量(5.2、15.6 g/kg)能显著减少MP小鼠的自主活动次数以及毛果芸香碱所致的发汗情况;高剂量(15.6 g/kg)能显著延长MP小鼠握力时间以及戊巴比妥钠诱导MP小鼠的睡眠时间,并显著增加阈下剂量戊巴比妥钠催眠MP小鼠入睡数量,降低MP小鼠的脂肪系数并升高MP小鼠的胸腺系数和脾系数。结论:ZRD能显著改善MP小鼠某些症状。AIM： The effect of Zhen Rong Dan Mixture （ZRD） on menopause syndrome was stud- ied. METHODS： Menopausal （MP） mice were used to study the effect of ZRD on autonomic activi- ty, the grip time, the sleeping time induced by pen- tobarbital sodium, threshold dose of pentobarbital sodium hypnosis experiment, pilocarpine sweating experiment and viscera index. RESULTS： Com- pared with the young mice, the independent activity time, grip strength time, sleeping time, thymus co- efficient and spleen coefficient were significantly de- creased by ZRD, while the khan point and fat coeffi- cient were significantly increased by ZRD. The 5.2 or 15.6 g/kg ZRD could significantly restrain the autonomic activity and reduce the pilocarpine induced sweating in MP mice. Treatment of MP mice with ZRD at dose of 15.6 g/kg could increase the grip time, prolong the sleeping time induced by pen- tobarbital sodium and improve the number of sleep- ing with the under-threshold dose of pentobarbital sodium. Further 15.6 g/kg ZRD reduced the fat in- dex and increased the spleen index and the thymus index in MP mice. CONCLUSION： ZRD can re- lieve some symptoms of climacteric syndrome.

关 键 词：贞蓉丹合剂 更年期综合征 药效学 实验研究 

分 类 号：R285.5[医药卫生—中药学] R932[医药卫生—中医学]