利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析  

An Analysis of Species Identification and Genetic Divergence of Drooping Wildrye( Elymus nutans) and Siberian Wildrye( E. sibiricus) by SSR Markers

作　　者：张成林[1] 郭志慧[1] 张新全[1] 白史且[2] 张昌兵[2] 李平 马啸[1] 

机构地区：[1]四川农业大学动物科技学院,成都611130 [2]四川省草原科学研究院,成都611731 [3]四川省甘孜藏族自治州畜牧科学研究所,康定626000

出　　处：《植物遗传资源学报》2016年第3期416-422,共7页Journal of Plant Genetic Resources

基　　金：国家牧草产业技术体系项目(CARS-35-05);四川省科技支撑计划(2013ZC1635;2015SZ0200)

摘　　要：垂穗披碱草(Elymus nutans)和老芒麦(Elymus sibiricus)皆为花序下垂类披碱草属物种,高海拔地区的E.sibiricus的部分变异类型也具有小穗紧密排列等特征,与E.nutans在形态学性状上具有较多的交叉,造成野外种质资源采集时进行直接的田间鉴定存在困难。本研究利用12对小麦族SSR引物对8份垂穗披碱草和10份老芒麦种质进行遗传变异和物种鉴定分析,UPGMA聚类分析表明供试材料明显可依据物种差异划分成两大类,主向量分析(PCo A)与聚类分析的结果保持一致。种质间遗传相似系数分析和分子方差分析(AMOVA)也表明种间变异远高于种内。另外,本研究筛选出3对引物,ESGS79和ESGS155能够在垂穗披碱草材料中扩增出特异性条带,Xgwm311能够在老芒麦材料中扩增出特异性条带,这3对引物能够作为区分垂穗披碱草和老芒麦的依据,为野外种质资源的收集以及田间育种工作提供指导。Elymus nutans and Elymus sibiricus,common species of Elymus genus of Triticeae tribe,are both tallgrowing,perennial bunchgrass with drooping inflorescence. When collecting wild germplasm in field survey,there are some difficulties in rapid and accurate identification of the two similar species due to some overlapped morphological characters such as dense spikelets in some E. Sibiricus collections from upper elevations. We used SSR primers from different sources to characterize genetic variance on a total of 18 accession of E. nutans and E. sibiricus. The 12 selected primers produced 152 well defined bands,and the percentage of polymorphism was 88. 6%. Two main clusters corresponded exactly to two species are shown in an arithmetic average（ UPGMA） dendrogram,which is in agreement with the results of principal coordinate analysis（ PCo A） analysis. The analysis of molecular variance（ AMOVA） and the t-test of Dice coefficients among accessions revealed genetic divergence between the two species was much higher than that within species. Furthermore,we screened out 3 primers,in which ESGS79 and ESGS155 are able to amplify the specific bands to E. nutans accession,while Xgwm311 could amplify specific bands to E. sibiricus. These primerswill provide guidance and convenience for the wild germplasm collection and breeding.

关 键 词：老芒麦 垂穗披碱草 SSR标记 遗传变异 物种鉴定 

分 类 号：S543.9[农业科学—作物学]