水稻雄性不育突变体D63的育性基因遗传分析与精细定位  

Genetic Analysis and Molecular Mapping of the Rice Male Sterile Mutant D63

作　　者：焦仁军[1] 朱柏羊 钟萍[1] 王迁[1] 廖婷婷[1] 王平荣[1] 邓晓建[1] 

机构地区：[1]四川农业大学水稻所,成都611130

出　　处：《植物遗传资源学报》2016年第3期529-535,共7页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31171533;31471473;91335107);四川省科技计划(2012NZ0027;2014JY0056)

摘　　要：利用化学诱变剂甲基磺酸乙酯(EMS)处理籼稻品种冈46B获得雄性不育突变体D63,并对该突变体进行表型鉴定、遗传分析和基因定位。结果显示D63突变体花药瘦小呈乳白色,花药内完全无花粉粒,属于无花粉型雄性不育。与野生型亲本冈46B相比,D63突变体成熟期株高降低了13.7%,穗伸出度减少了266.7%,自交结实率为0,其他农艺性状无显著差异。遗传分析表明该不育性状受1对隐性核基因控制,该突变基因定位于第2号染色体长臂靠近着丝粒区域In Del标记J2和J4之间,与J2和J4的遗传距离分别为0.2 c M和0.1 c M,该定位区间的物理距离为105.8 kb。候选基因分析结果表明,D63突变体在编码分泌性成束糖蛋白基因LOC_Os02g28970编码区第1580位碱基A突变为C,使编码蛋白的氨基酸序列第527位组氨酸(His)突变为脯氨酸(Pro)。D63突变体与已报道的mtr1突变体表型上不同之处主要是后者花药含有败育花粉粒,二者表型上的差异可能是由于LOC_Os02g28970基因序列突变位点不同,以及它们分别属于籼、粳亚种2个不同遗传背景所致。In this study,the male sterile mutant D63 was isolated from the indica cultivar G46 B through ethyl methanesulfonate（ EMS） mutagenesis. Furthermore,phenotypic characterization,genetic analysis and gene mapping of the mutant were performed. As result,anthers of the D63 mutant were smaller,thiner and milk white,and there was no pollen in its pollen sac,which belonged to non-pollen type of male sterility. During the mature period,compared with the wide-type parent G46 B,plant height and panicle exsertion of D63 reduced by 13. 7% and 266. 7%,respectively,and selfing seed setting rate was 0. Genetic analysis indicated that male sterility of the D63 mutant was controlled by one pair of recessive nuclear gene. The sterile gene was finally mapped between the In Del markers J2 and J4 on the long arm of rice chromosome 2. The genetic distances from the sterile gene to the two In Del markers were 0. 2 c M and 0. 1 c M,respectively,and the physical length of this region was 105. 8 kb. Sequenceing analysis of the candidate genes in the mutant and its wild-type revealed a single-nucleotide A-to-C mutation existed in the D63 mutant at position 1580 in coding region of the LOC_Os02g28970 gene for secretory fasciclin glycoprotein,which resulted in an amino acid change from His at position 527 to Pro in the encoded product. The D63 mutant had completely no pollen grain in its anthers,but the reported mtr1 mutant had abortive pollen grains. The differenent pheno-types of the two mutants may be caused by different mutational sites of the base sequence of LOC_Os02g28970gene,or different genetic backgrounds of the two subspecies,because the two mutant belonged to indica and japonica subspecies,respectively.

关 键 词：水稻 雄性不育 核不育 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]