栽培一粒小麦3AA30中抗白粉病基因的鉴定及分子标记定位  

Identification and Molecular Mapping of the Powdery Mildew Resistance Gene in Einkorn Wheat( Triticum monococcum L. ) Accession 3AA30

作　　者：刘婉辉 董宏图[1] 李映辉[1] 耿妙苗 李峰[1] 刘鑫[1] 解超杰[1] 孙其信[1] 

机构地区：[1]中国农业大学农学与生物技术学院/农业生物技术国家重点实验室/农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室/教育部作物杂交优势研究与利用重点实验室,北京100193

出　　处：《植物遗传资源学报》2016年第3期536-540,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31271708);国家"973"项目(2009CB118300)

摘　　要：栽培一粒小麦是普通小麦的近缘种,遗传多样性丰富,蕴含丰富的抗病基因,是小麦抗病性改良的重要资源。本文对栽培一粒小麦抗白粉病材料3AA30的抗白粉病基因进行了遗传分析和分子标记定位。结果表明,3AA30中含有一个隐性抗白粉病基因,暂命名为ml3AA30,找到了5个与该基因连锁的SSR分子标记Xgwm6、Xcfd39、Xcfa2185、Xcfa2141、Xcfa2155及2个STS标记Xmag2170、Xmag1491,并构建了ml3AA30的遗传连锁图,将该基因定位在小麦5A染色体长臂上。本研究为小麦抗病育种提供了新的抗源材料。The einkorn wheat contains abundant resistance genes and is one of important resources for common wheat cultivar improvement. An einkorn wheat accession 3AA30 was proved to be resistant to wheat powdery mildew in Beijing. Genetic analysis showed that 3AA30 carried a recessive resistance gene,temporally designated as ml3AA30. Five SSR markers（ Xgwm6,Xcfd39,Xcfa2185,Xcfa2141,and Xcfa2155） and two STS markers（ Xmag2170 and Xmag1491） on chromosome 5AL of wheat were found linked to ml3AA30 by bulked segregant analysis,and a genetic map of ml3AA30 was established. Based on the above result,powdery mildew resistance gene ml3AA30 was located on the long arm of wheat chromosome 5A. This result provided new materical for wheat powdery mildew resistance breeding program.

关 键 词：栽培一粒小麦 3AA30 抗白粉病基因 分子标记 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]