陆地棉bZIP转录因子响应非生物胁迫表达谱分析  

Expression Profiling of Cotton( Gossypium hirsutum L. )bZIP Genes Responsive to Abiotic Stresses

作　　者：李月[1] 许朋斐 刘超[1] 代培红[1] 葛杰[1] 曲延英[1] 刘晓东[1] 

机构地区：[1]新疆农业大学农学院/农业生物技术重点实验室,乌鲁木齐830052

出　　处：《植物遗传资源学报》2016年第3期562-569,共8页Journal of Plant Genetic Resources

基　　金：国家"863"计划(2012AA101108-05);国家自然科学基金(31470289);新疆农业大学校前期课题资助(XJAU201311)

摘　　要：低温、干旱和高盐是影响棉花生长发育和产量的重要限制因素。b ZIP转录因子在植物非生物胁迫反应中起重要作用。本研究利用生物信息学的方法从陆地棉中鉴定了24个b ZIP转录因子基因,命名为Ghb ZIP1~Ghb ZIP24。系统进化树分析表明,这24个家族成员主要聚集在A、B、C、D、E、G、I、S这8类亚家族。通过RT-PCR的方法分析了棉花（Gossypium hirsutum L.）Ghb ZIPs基因在高盐（200 mmol/L Na Cl）、干旱、4℃低温等非生物胁迫处理下的表达模式。结果表明,19个基因响应高盐胁迫、11个基因对干旱胁迫有应答反应,15个基因有冷胁迫应答。此外,有4个基因（Ghb ZIP4、Ghb ZIP7、Ghb ZIP21和Ghb ZIP23）在3种处理下均有应答反应。以上研究结果表明,Ghb ZIPs在陆地棉的非生物胁迫适应过程中可能具有重要的作用。本研究为进一步探索棉花b ZIP转录因子在抗逆反应中的重要作用和利用基因操作手段提高棉花抗逆性提供了重要信息。Low temperature,drought and salinity are major abiotic stresses that influence survival,productivity of cotton. b ZIP transcription factor genes play significant roles in the response to abiotic stresses. In this study,twenty-four b ZIP genes,named as Ghb ZIP1-Ghb ZIP24,were identified in cotton（ Gossypium hirsutum L.） by bioinformatic approach. Phylogenetic analysis revealed that the isolated cotton b ZIPs genes could be classified into 8 groups： A,B,C,D,E,G,I,S. Semi-quantitative RT-PCR were employed to reveal the expression profiling of 24 Ghb ZIPs under different abiotic stresses（ 200 mmol / L Na Cl,drought,4 ℃） treatments. The results showed that the expression of 19 Ghb ZIP genes and 11 Ghb ZIP genes were up-or down-regulated by salinity and drought stresses respectively. 15 Ghb ZIP genes were up-or down-regulated by cold treatments. Moreover,four genes including Ghb ZIP4,Ghb ZIP7,Ghb ZIP21 and Ghb ZIP23 were response to all above treatments. The results suggested that Ghb ZIPs might play important roles in upland cotton adaptation to abiotic stress. Taken together,our data provided valuable information for further exploring the roles of the cotton b ZIPs genes in response to stress and will eventually lead to the development of genetically manipulated cotton varieties with improved stress tolerance.

关 键 词：棉花 bZIP转录因子 非生物胁迫 RT-PCR 表达分析 

分 类 号：S562[农业科学—作物学]