牛支原体NM2012株致病性研究  

Study on Pathogenicity of Mycoplasma bovis NM2012 Strain

作　　者：高航飞[1] 李建[1] 牛同州[1] 涂明亮 李雪峰[2] 纪燕 赵永春 申之义[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]金宇生物技术股份有限公司,内蒙古呼和浩特010030 [3]内蒙古包头市农牧业局,内蒙古包头014010

出　　处：《畜牧与饲料科学》2016年第4期18-23,26,共7页Animal Husbandry and Feed Science

摘　　要：[背景]牛支原体是牛呼吸道疾病综合征的重要病因之一,可以引起牛的多种呼吸道疾病,给养殖业带来巨大的经济损失。接种疫苗是预防牛支原体感染的最有效的手段。[目的]通过人工感染牛体试验,了解牛支原体NM2012株的致病性,为后续疫苗研究提供生产用菌种。[方法]将牛支原体NM2012株第6代、第12代、第20代菌株分别经人工气管注射感染2周龄犊牛,攻毒后连续观察27 d,记录攻毒后犊牛的临床症状,在感染后第14天,从各攻毒组分别选取3头犊牛、空白对照组选取2头犊牛进行扑杀,第27天将剩余实验犊牛处死,观察肉眼病理变化和组织学病理变化。[结果]3个代次的NM2012菌株均对犊牛具有致病性,可引起比较典型的牛支原体肺炎症状和病理变化。[结论]NM2012株6-20代仍然具有对牛的致病性,可以作为今后疫苗研究的潜在菌种。[Background] Mycoplasma bovis is a major aetiological agent of bovine respiratory disease complex（BRDC） which causes a variety of respiratory diseases of bovine and results in huge economic losses in livestock farming. Vaccination is the most efficient way to prevent the infections causes by Mycoplasma bovis. [Objective] To understand the pathogenicity of Mycoplasma bovis NM2012 strain by artificial infection tests and to provide potential strains for vaccine development. [Methods] Two-weekold calves were artificially infected by endotracheal inoculation with F_6, F_（12） and F_（20）of Mycoplasma bovis NM2012 strain,respectively. The clinical symptoms of the challenged calves were monitored for consecutive 27 days. At the 14-（th） day postchallenge, 3 calves chosen from each challenged group and 2 calves chosen from control group were killed, respectively. At the 27-（th） day postchallenge, all the other calves were killed. The pathological changes of the killed calves were observed by naked eyes and histologically analyzed. [Results] The F_6,F_（12） and F_（20） of Mycoplasma bovis NM2012 strain exhibited pathogenicity to calves and causes typical symptoms and pathological changes of Mycoplasma bovis pneumonia. [Conclusion] The results suggest that the F_6 to F_（20）of Mycoplasma bovis NM2012 strain remains infectious to bovine, and Mycoplasma bovis NM2012 strain may serve as a candidate strain for vaccine development.

关 键 词：牛支原体病 牛支原体 人工感染 犊牛 致病性 

分 类 号：S852.62[农业科学—基础兽医学]