一株产酯酶菌的分离鉴定及其酶学性质研究  

Isolation and Identification of An Esterase-Producing Strain and Its Enzymatic Properties

作　　者：段俐[1] 赵一[1] 王崇慧[1] 王丽丽[1] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《化学与生物工程》2016年第5期20-25,共6页Chemistry & Bioengineering

基　　金：国家863计划资助项目(2014AA022102)

摘　　要：从土壤中分离得到一株产酯酶菌,经16S rDNA序列测定,属于嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia),命名为S.mal SF-H。对该菌株产酶的诱导效应及其酶学性质进行了研究。结果表明,培养基中加入5g·L^(-1)柠檬酸三正丁酯塑化剂,可以有效诱导该菌株产生酯酶,所产酯酶对短链脂肪酸酯的水解活性有明显底物特异性和差异性。以对硝基苯乙酸酯为底物时,酶液表现出良好的耐温性,最适温度为80℃,且70℃保温2h的相对酶活力为95%,最适pH值为9.0,在pH值为8.0时稳定性最好;以对硝基苯丁酸酯为底物时,酶的最适温度为70℃,70℃保温2h的相对酶活力为51%,最适pH值为8.0,在pH值为8.0~10.0时稳定性最好。所产酯酶的耐溶剂性表现基本一致,在66%丁醇和66%乙醇中相对酶活力均约为60%。An esterase-producing strain was isolated from the soil.It was identified as Stenotrophomonas maltophilia by 16S rDNA sequence analysis and named as S.mal SF-H.The inductive effects of esterase producing and the enzymatic properties of the strain were studied.Results showed that adding 5g·L^（-1） tributyl citrate（TBC）to the medium could effectively induce the strain to produce esterases.The esterases had significant substrate specificity and diversity on hydrolytic activity among short-chain aliphatic esters.When using p-nitrophenyl acetate as a substrate,S.mal SF-H exhibited great temperature tolerance.The optimum temperature was 80 ℃,and the relative enzyme activity was 95% after water bath at 70 ℃ for 2h,the optimum pH value was 9.0,and enzyme stability was the best at pH=8.0.When using p-nitrophenyl butyrate as a substrate,the optimum temperature was 70℃,and the relative enzyme activity was 51% after water bath at 70℃ for 2h,the optimum pH value was 8.0,and enzyme stability was the best at pH=8.0～10.0.However,the solvent resistance for all esterases were similar,the relative enzyme activities were about 60% in 66% butanol or 66% ethanol solvents.

关 键 词：酯酶 柠檬酸三正丁酯 16S RDNA 嗜麦芽寡养单胞菌 

分 类 号：Q556[生物学—生物化学]