IFIT1在放射损伤与感染应激中表达的特点和意义  

Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress

作　　者：郭晓姝[1,2] 郝玉徽[1] 粟永萍[1] 王军平[1] 王崧[1] 许杨[1] 

机构地区：[1]第三军医大学全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038 [2]长治医学院生理教研室,山西长治046000

出　　处：《局解手术学杂志》2016年第5期323-326,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：“十二五”国家科技支撑计划项目(2013BAK03B05-02);国家重点实验室开放课题(SKLKF201321);国家重点实验室自主研究课题(SKLZZ201503)

摘　　要：目的探讨IFIT1(interferon-induced protein with tetratricopetide repeats 1)在放射损伤与感染应激中表达的特点和意义。方法采用5μg/mL的脂多糖(LPS)刺激Raw264.7、3T3和10T1/2细胞株5 h后,收集细胞,提取RNA,行RT-PCR观察IFIT1的表达情况,同时设定空白对照组。另外,将20只C57/BL6小鼠随机分为4组,采用12 Gray60Co一次性全身照射,分别于0 h、1 h、4 h和12 h脱颈处死取肝组织,用Western blot测定肝组织IFIT1表达变化。结果 Raw264.7、3T3和10T1/2细胞株经LPS刺激5 h后,均能诱导IFIT1表达,空白对照组表达为阴性。C57/BL6小鼠放射性损伤后,1 h IFIT1/Actin开始增高,12 h达最高,呈上升趋势,差异有极显著性统计学意义(P<0.01)。结论 LPS可刺激多种细胞株表达IFIT1,提示IFIT1可能参与创伤后毒血症的发生和发展;放射损伤早期能引起小鼠肝组织IFIT1的显著增高。Objective To explore characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress. Methods RNA was extracted from Raw264. 7 cell,3T3 cell and 10T1 /2 cell after 5 hours stimulated with 5 μg / mL LPS. At the same time,to set up normal control group（ untreated by LPS）,and RNA of IFIT1 was detected by RT-PCR. Totally 20 C57 / BL6 mice were randomly divided into 4 groups,namely 0 h group,1 h group,4 h group and 12 h group. The mice were given 12Gray60 Co full-body exposure once,then liver IFIT1 was detected by western blot. Results Stimulated with LPS for 5 hours,IFIT1 was induced expression in Raw264. 7 cell,3T3 cell and 10T1 /2 cell. The expression of normal control group was negative. The level of IFIT1 / Actin increased significantly 1 hour after radiation injury,and it reached the peak 12 hours after radiation injury（ P 0. 01）. Conclusion LPS can stimulated a variety of cell lines expressed IFIT1,prompting that IFIT1 may participate in the occurrence and development of post-traumatic toxemia. IFIT1 of liver tissue increased significantly during the early stage in radiation mice.

关 键 词：放射性损伤 脂多糖 IFIT1 糖皮质激素受体 毒血症 

分 类 号：R631.1[医药卫生—外科学] R146[医药卫生—临床医学]