颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究  

Granule-based anaerobic biofilm enhances propionic acid degradation under high H_2 partial pressure

作　　者：徐恒[1] 汪翠萍[1] 颜锟 孟尧[1] 刘晓吉[1] 王凯军[1] 

机构地区：[1]清华大学环境学院环境模拟与污染控制国家重点联合实验室,北京100084

出　　处：《中国环境科学》2016年第5期1435-1441,共7页China Environmental Science

基　　金：国家科技支撑计划课题(2014BAC27B01);水体污染控制与治理科技重大专项(2012ZX07212-001)

摘　　要：为了改善氢辅助型原位沼气提纯系统中高氢分压对丙酸降解的抑制,考察了不同颗粒型厌氧生物膜(有无载体、导电和非导电载体)培养初期和末期的丙酸降解性能;并通过微生物形态和群落分析,探讨了颗粒型生物膜丙酸降解机制.结果表明,导电碳毡厌氧生物膜和厌氧颗粒污泥能有效改善高氢分压下丙酸降解抑制问题.其最大丙酸降解速率分别达到2.2,1.2mmol/(L·h).碳毡厌氧生物膜可能主要通过产酸细菌(Thermovirga、Levilinea、Syntrophomonas属)和产甲烷古菌(Methanosaeta属)的电子直接传递(DIET)途径实现丙酸的降解;而厌氧颗粒污泥降解丙酸的途径可能主要依靠产酸细菌(Syntrophobacter属)与嗜氢型甲烷菌(Methanolinea、Methanobacterium属)的共生营养代谢过程.Different types of granule-based anaerobic biofilm（with and without carriers;conductive and non-conductive carriers） were adopted to alleviate the inhibition of propionic acid degradation under high H_2 partial pressure.And their performance was monitored and compared,at the beginning and end of biofilm cultivation.Moreover,microbial SEM images and community composition were investigated to analyze mechanisms involved in propionic acid degradation.The results showed that both conductive carbon-felt based biofilm and anaerobic granules were effective at promoting propionic acid degradation under high H_2 partial pressure,with maximum rate of 2.2 and 1.2mmol/h respectively.Propionic acid seemed to be degraded mainly via direct interspecies electron transfer（DIET） between acidogenic bacteria（Thermovirga,Levilinea,Syntrophomonas） and methanogens（Methanosaeta） for carbon-felt based biofilm.Nevertheless,degradation of propionic acid by anaerobic granules was possibly attributed to syntrophic operation of acidogenic bacteria（syntrophobacter） and methanogens（Methanolinea,Methanobacterium）.

关 键 词：颗粒型生物膜 导电载体 高氢分压 丙酸降解 厌氧颗粒污泥 

分 类 号：X705[环境科学与工程—环境工程]