同步辐射微区X射线荧光光谱法原位分析蚯蚓中K,Ca,Cu,Zn和Pb  

Distribution Analysis of K,Ca,Cu,Zn and Pb in Earthworm by In Situ Synchrotron Radiation Micro X-Ray Fluorescence

作　　者：袁静[1,2,3] 罗立强[2,3] 

机构地区：[1]中国地质调查局南京地质调查中心,南京210016 [2]国家地质实验测试中心,北京100037 [3]中国地质大学(北京),北京100083

出　　处：《分析化学》2016年第5期792-798,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.20775018;41201527);国家高新技术研究发展计划863项目(No.2007AA06Z124);中国地质调查局地质大调查项目(No.12120113015200);国家科技专项"汶川地震断裂带科学钻探工程"0008课题和0003课题第四专题的联合资助~~

摘　　要：为了研究重金属元素在蚯蚓体内的富集和存储机制,使用同步辐射微区X射线荧光光谱法(μ-SRXRF)研究了K,Ca,Cu,Zn和Pb元素在生长于南京栖霞山铅锌矿区附近菜园土壤的蚯蚓体内的分布特征,发现Pb主要富集在蚯蚓后部消化道周围的区域,Zn,Cu在蚯蚓后部的分布规律与Pb类似,推测后部消化道周围区域的分布是蚯蚓为了阻断毒性重金属元素威胁而特有的富集和存储方式。5种元素的相关性表明,Pb和Zn相关性最高,存储方式最为相似,Pb与K和Ca在蚯蚓后部的分布呈显著正相关,说明毒性元素Pb在蚯蚓体内的富集和存储过程可能伴随着其它元素的吸收。本研究表明,μ-SRXRF在原位微区分析蚯蚓样品的元素空间分布方面具有很大优势,而进一步开展土壤及蚯蚓中Pb的形态研究,是研究重金属胁迫下蚯蚓解毒机制的重要前提。To study the accumulation and storage mechanism of heavy metals in earthworms, synchrotron radiation micro X-ray fluorescence （μ-SRXRF） was used to determine element distribution characteristics of K, Ca, Cu, Zn and Pb in earthworms living in garden around Qixiashan lead-zinc mine of Nanjing. The imaging of these elements showed that Pb primarily accumulated in the region around the posterior alimentary canal. There were also Zn and Cu signals in the anterior and posterior part of earthworm, besides the distribution characters of Zn and Cu in the posterior part of earthworm were the same as that of Pb. The findings indicated that the distribution characters in the region around the posterior alimentary canal was a special accumulation and storage mechanism in earthworms for cutting the threat of toxic heavy metals. The high relationship between Pb and Zn distribution indicated that the two elements had a very similar pathway. In this study, we proved that μ-SRXRF had a great advantage in spatial distribution analysis of elements in earthworm samples. A further research focusing on Pb speciation in soil and earthworms is needed for understanding the detoxification mechanism in earthworms under heavy metal stress.

关 键 词：同步辐射微区X射线荧光光谱法 蚯蚓 原位分析 铅 存储机制 

分 类 号：X835[环境科学与工程—环境工程] O657.34[理学—分析化学]