基于立方体纳米氧化亚铜修饰的安培型葡萄糖生物传感器的制备及性能研究  

A Highly Sensitive Amperometric Glucose Biosensor Based on Nano-cube Cu_2O Modified Glassy Carbon Electrode

作　　者：马真真[1] 余会成[1,2] 吴朝阳[1] 吴燕[1] 肖福兵[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,化学化工学院,长沙410082 [2]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西高校食品安全与药物分析化学重点实验室,南宁530006

出　　处：《分析化学》2016年第5期822-827,共6页Chinese Journal of Analytical Chemistry

基　　金：广西自然科学基金(No.2015GXNSFAA139040);国家自然科学基金(Nos.21175037,21277042)资助~~

摘　　要：将合成的立方体纳米氧化亚铜用于修饰玻碳电极,在其上固定葡萄糖氧化酶,构建了高灵敏的安培型葡萄糖生物传感器。采用X射线衍射(XRD)、扫描电镜(SEM)对合成的立方体纳米氧化亚铜及其修饰电极进行了表征。结果表明,合成的纳米氧化亚铜为均匀的立方体形状。采用循环伏安法(CV)、交流阻抗谱(EIS)、差分脉冲伏安法(DPV)及计时电流法(CA)考察了修饰电极的电化学行为。在含0.1 mmol/L葡萄糖的磷酸盐缓冲溶液(p H 7.4)中研究了立方体纳米氧化亚铜修饰电极的循环伏安(CV)响应,实验结果表明,此修饰电极对葡萄糖显示出良好的电催化性能。DPV响应电流与葡萄糖的浓度在5.0×10^(-6)~4.0×10^(-3)mol/L范围内呈良好的线性关系,线性相关系数R^2=0.9983,检出限为6.8×10^(-7)mol/L(S/N=3)。CA实验结果表明,尿酸、抗坏血酸、D-果糖对传感器不产生干扰。本传感器具有较好的重现性和稳定性,可用于实际样品中葡萄糖的检测。A highly sensitive amperometric glucose biosensor was fabricated by using the synthesized nano- cube copper （I） oxide （Cu2O）. The synthesized nano-Cu2O and the modified electrode were characterized using X-ray diffraction （XRD）, scanning electron microscopy （SEM）. The results showed that the obtained samples were nano-cube Cu2O. The electrochemical properties of the modified electrode were investigated by cyclic voltammetry （CV）, differential pulse voltammetry （DPV）, electrochemical impedance spectroscopy （ EIS）, and chronoamperometry （CA）. The CV behavior of nano-cube Cu2O modified sensor was examined in 0.1 mol/L PBS （pH 7.4） containing 0.1 mmol/L glucose. The experimental results showed the prepared sensor presented remarkably enhanced electrocatalytie activity towards glucose detection. Linear responses of the sensor to glucose were observed for gulcose concentrations ranging from 5. 0 × 10-6 mol/L to 4.0× 10-3 mol/L （ r2 = 0.9983 ）, with a detection limit of 6.8 × 10-7 mol/L （ S/N = 3 ）. The CA results showed that the interference from potential interfering species such as uric acid, ascorbic acid, and D fructose could be ignored. In addition, the developed sensor exhibited good repeatability and stability, and was successfully applied to the determination of glucose in real urea samples from diabetics.

关 键 词：立方体纳米氧化亚铜 葡萄糖氧化酶 葡萄糖 电催化 生物传感器 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]