中国结核分枝杆菌五种特异性抗原人B细胞表位多态性分析  

Analysis on the polymorphisms of human B cell epitopes of 5 specificity antigens in Mycobacterium tuberculosis in China

作　　者：徐永娟[1] 李马超[2] 陈双双[1] 肖诗琪[3] 刘海灿[2] 赵秀芹[2] 吴移谋[1] 万康林[2] 

机构地区：[1]湖南省衡阳市南华大学病原生物学研究所,421000 [2]传染病预防控制国家重点实验室传染病诊治协同创新中心中国疾病预防控制中心传染病预防控制所 [3]中国农业大学生物技术国家重点实验室微生物与免疫学系

出　　处：《中华结核和呼吸杂志》2016年第5期372-377,共6页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家科技重大专项课题（2013ZX10003006-002-001）;国家自然科学基金（81401647）

摘　　要：目的 研究我国结核分枝杆菌复合群（MTBC）抗原mpt83、mpt70、IpqH、pstS3、groES及其人B细胞表位的多态性。方法从中国疾病预防控制中心传染病所结核室菌株库随机选取我国的179株MTBC临床分离株，培养后提取DNA，基因扩增并测序，根据H37Rv标准序列和免疫表位数据库（IEDB）的抗原表位信息，比较、分析各基因B细胞表位序列的变异情况，最后用MEGA6软件计算各基因的非同义突变率（dN）、同义突变率（ds）及其比值。结果在179株MTBC菌株中，mpt83基因有7个单核苷酸多态性（SNP）位点，该基因及其表位区、非表位区的dN／dS分别为0．88、0．34和0．14；mpt70基因有1个SNP位点，为非同义突变；IpqH基因及其B细胞表位区dN／dS均为0．38，非表位区dN、ds均为0；pstS3基因及B细胞非表位区的dN／dS分别为1．74和1．40；groES基因未发现突变位点。结论结核分枝杆菌mpt83、IpqH、pstS3的基因序列及B细胞表位具有较高的多态性，而mpt70和groE基因B细胞表位较保守。Objective To study the diversities of human B cell epitopes of five proteins （Mpt83, Mpt70,LpqH, PstS3,GroES） in Mycobacterium tuberculosis isolates from China. Methods We selected 179 strains isolated from patients with tuberculosis in different regions of China, and cultured these strains by L-J medium. The gene sequences of these 5 proteins were amplified and sequenced by PCR. The variations （ single nueleotide polymorphisms, SNP） of the DNA sequences were compared and analyzed according to the immune epitope database （ IEDB ）. The synonymous mutation rate （ dS）, non-synonymous mutation rate （dN） and dN/dS values were calculated with MEGA6 software. Results Among the 179 clinical MTBC isolates, 7 SNP mutations were found in the mpt83gene. The dN/dS value of the whole mpt83 gene was 0. 88. One SNP mutation was found in the mpt70 gene. The dN/dS values of the whole pstS3 gene and non- B epitopes areas were 1.74 and 1.40 respectively. But no mutation was found in the groES gene. Conclusion The mpt83 ,lpqH and pstS3 genes of 179 MTBC strains contain gene SNPs and human B cell epitopes diversities, while the coding gene and human B cell epitopes of protein mpt70 and groES were conservative.

关 键 词：分枝杆菌 结核 表位 B淋巴细胞 多态性 单核苷酸 

分 类 号：R378.911[医药卫生—病原生物学]