纯镁超声微弧氧化-植酸-载锌复合膜层的细胞相容性  

Biocompatibility of the composite coating on pure magnesium treated by ultrasonic micro arc oxidation,phyticacid and zinc

作　　者：肖月[1] 陈洪伟[1] 李慕勤[1] 王爽[1] 刘明达[1] 魏方红[1] 

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007

出　　处：《中国体视学与图像分析》2016年第1期117-122,共6页Chinese Journal of Stereology and Image Analysis

基　　金：佳木斯大学研究生科技创新项目(LM2015_079);国家自然科学基金(No.31370979);黑龙江省高校创新团队建设计划项目;生物医用材料及其表面改性(2012TD010)

摘　　要：目的为调控医用纯镁的降解速度和赋予材料表面生物活性,对其表面进行超声微弧氧化(UMAO)、植酸、载锌复合处理,研究不同处理对成骨细胞生物相容性影响,为纯镁在临床上应用提供依据。方法以纯镁UMAO为对照组(A组),纯镁UMAO-植酸(B组)和纯镁UMAO-植酸-载锌(C组)为实验组。通过碱性磷酸酶(ALP)和Cell Counting Kit(CCK-8)试剂盒、扫描电镜、激光共聚焦显微镜等方式检测不同处理膜层细胞相容性。结果 ALP和CCK8测定的吸光率,B组大于A组,C组高于B组,膜层表面细胞相容性随时间呈递增趋势,其中纯镁UMAO-植酸-载锌复合膜层具有最优的成骨细胞相容性。据统计学分析,A、B、C三组结果均具备统计学意义。结论 UMAO-植酸-载锌复合处理后膜层的细胞相容性最好,UMAO-植酸处理次之,UMAO组最低。Objective In order to control the degradation rate and the surface biological properties of medical pure magnesium,the pure magnesium was treated by ultrasonic micro arc oxidation（ UMAO）,phytic acid and zinc. Studying the effect of various treatment on the biocompatibility of osteoblasts,and providing a theoretical basis for clinical application of pure magnesium. Methods The UMAO-treatedpure magnesium was designated as the control group（ group A）,and the magnesium UMAO-phytic acid（ group B） and magnesium UMAO-phytic acid-zinc loaded（ group C） were designated as experimental groups. The biocompatibility of coating was assessed through ALP,CCK8,scanning electron microscopy and laser confocal microscopy. Results The absorbance rate of ALP and CCK8 of the group B was higher than that of group A,and that of the group C was higher than the group B. The biocompatibility of the coating surface was increasing with the time increase,among them,the pure magnesium UMAO-phytic acid-zinc composite had the best compatibility of osteoblasts. The statistical analysis showed that the differences among the groups A,B and C were significant. Conclusions The cell compatibility of the composite coating treated with UMAO-phytic acid-zinc is the best,followed by the UMAO-phytgic acid group,and least of the UMAO group.

关 键 词：微弧氧化 纯镁 植酸 锌离子 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]