S-HPM辐射对大鼠睾丸TLR表达的影响  

Effects of S- HPM radiation on the expression of TLR in rat testis

作　　者：吴惠[1] 孟宇宏[1] 王德文[2] 宁浩勇[1] 刘肖[1,2] 谢一帆[2] 王少霞[2] 李杨[2] 徐新萍[2] 左红艳[2] 彭瑞云[2] 

机构地区：[1]海军总医院病理科,北京100048 [2]军事医学科学院放射与辐射医学研究所研究室,北京100850

出　　处：《中国体视学与图像分析》2016年第1期130-136,共7页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金资助项目(No.81302397)

摘　　要：目的探讨S波段高功率微波(S band high power microwave,S-HPM)辐射对大鼠睾丸Toll样受体(Toll-like receptor,TLR)表达的影响。方法 60只雄性Wistar大鼠经0 m W/cm2、30 m W/cm2和100 m W/cm2的S-HPM辐射20 min,通过TUNEL方法检测辐射后6 h、3 d、7 d和14d睾丸生精细胞的凋亡。采用Real-time PCR和Western blotting检测辐射后睾丸组织TLR2、TLR3、TLR4和TLR5的mRNA和蛋白水平的表达变化。结果经30 m W/cm2和100 m W/cm2的S-HPM辐射后,生精细胞凋亡率随时间延长而增加,100 m W/cm2组重于30 m W/cm2组(P<0.05);S-HPM辐射后6 h、3 d和7 d可见TLR2-5 mRNA表达不同程度的增加(P<0.05,P<0.01);辐射后7 d可见TLR2-5蛋白表达的增加,100 m W/cm2与30 m W/cm2组相比无显著差异。结论 S-HPM辐射可致睾丸生精组织凋亡,TLR2-5的表达上调,参与了微波辐射致生精细胞凋亡的病理生理过程。Objective To explore the expression of Toll- like receptor（ TLR） in rat testis induced by S- HPM（ S band high power microwave radiation）. Methods 60 male Wistar rats were radiated with 0,30 and 100 m W / cm2S- HPM for 20 min,and were sacrificed at 6 h,3 d,7 d and 14 d after exposure.Germ cells apoptosis was detected by TUNEL assay. TLR2- 5 expression was assessed by Real-time PCR and western blotting. Results After irradiated by 30 m W / cm2 and 100 m W / cm2S- HPM radiation for6 h- 14 d,the rate of apoptotic germ cells increased with time extending. The elevation in 100 m W / cm2 groups was more clearly than that of 30 m W / cm2group（ P 0. 05）. TLR2- 5 mRNA expressions increased at different degree at 6 h,3 d or 7 d（ P 0. 05,P 0. 01）. The protein level of TLR2- 5increased at 7 d（ P 0. 05）,but no significant differences were observed in 30 m W / cm2 groups and 100 m W / cm2 groups. Conclusions S- HPM may lead to the germ cells apoptosis and increase the TLR2- 5expression,which may mediate the pathophysiological process of germ cell apoptosis induced by microwave radiation.

关 键 词：S-HPM辐射 睾丸 TLR 凋亡 

分 类 号：R285.5[医药卫生—中药学]