ATP依赖型染色质重塑复合物在DNA双链断裂修复中的作用  

The roles of ATP-dependent chromatin remodeling complexes in DNA double-strand break repair

作　　者：周建杰[1] 陈学峰[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《生命科学》2016年第4期452-463,共12页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金面上项目(31471217)

摘　　要：DNA双链断裂修复缺陷易导致细胞基因组稳定性失衡、细胞发生癌变或死亡。真核生物主要通过同源重组和非同源末端连接两条途径来修复双链断裂。近年来发现多种ATP依赖型的染色质重塑蛋白复合物,包括RSC、INO80、Fun30、SWI/SNF和SWR1,直接参与了DNA双链断裂修复过程。它们主要通过调控DNA损伤检查点激活、断裂末端剪切及组蛋白H2AZ-H2B/H2A-H2B置换等重要步骤发挥功能。现以酿酒酵母中的研究为重点,综述主要ATP依赖型染色质重塑复合物在DNA双链断裂修复中的功能及作用机制。Defects in repair of DNA double-strand breaks （DSBs） can lead to genome instability, tumorigenesis or cell death. Eukaryotic cells employ two major pathways, homologous recombination （HR） and non-homologous end joining （NHEJ）, to repair DSBs. Recent studies have revealed that several ATP-dependent chromatin remodeling complexes, including RSC, INO80, Fun30, SWI/SNF and SWR1, play direct roles in DSB repair. These remodeling factors exert critical functions at multiple key steps in DSB repair, such as DNA damage checkpoint activation, end processing or H2AZ-H2B/H2A-H2B exchange. In this review, we summarized recent progresses on this topic with an emphasis on the roles and mechanisms of these chromatin remodeling factors in Saccharomyces cerevisiae.

关 键 词：DNA双链断裂 染色质重塑复合物 检查点 同源重组 非同源末端连接 

分 类 号：Q754[生物学—分子生物学]