江苏省粳稻品种抗稻瘟病基因型与穗颈瘟抗性分析  

Relacionship between rice blast resistance genotypes and neck blast resistance of Japonica rice in Jiangsu province

作　　者：王军[1,2] 宫丹妮 杨杰[1,2] 朱金燕[1,2] 范方军[1,2] 李文奇[1,2] 王芳权[1,2] 仲维功[1,2] 

机构地区：[1]江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心,江苏南京210014 [2]扬州大学江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009 [3]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《江苏农业学报》2016年第2期250-256,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑计划重大项目(2011BAD16B03);江苏省农业科技自主创新基金项目[CX(12)1003];国家自然科学基金项目(31400175);江苏省自然科学基金项目(BK20130725)

摘　　要：稻瘟病是江苏省粳稻的主要病害,以穗颈瘟的危害最为严重,选育抗病品种是防治该病害最有效的方法。为了明确江苏省粳稻穗颈瘟的主要抗病基因以及主要粳稻品种的抗稻瘟病基因型,利用稻瘟病抗性基因Pita、Pi-b、Pi-k^h、Pi-km的功能标记对2007-2013年江苏省审定的粳稻品种进行基因型检测。结果表明:65份审定的粳稻品种中同时含有3个稻瘟病抗病基因的品种6份,占审定品种的9.2%;同时含有2个稻瘟病抗病基因的品种23份,占审定品种的35.4%;含有1个稻瘟病抗病基因的品种27份,占审定品种的41.5%;不含有这4个抗病基因的品种9份,占审定品种的13.9%。2014年穗颈瘟接种鉴定结果表明:含有3个抗病基因的6份品种均表现为抗穗颈瘟;含有2个抗病基因的品种中19份品种抗穗颈瘟,4份品种感穗颈瘟;含有1个抗病基因的品种中12份品种抗穗颈瘟,15份品种感穗颈瘟;不含有这4个抗病基因的品种中1份品种抗穗颈瘟,8份品种感穗颈瘟。相关性和回归分析结果表明:稻瘟病抗性基因Pi-ta、Pi-b、Pi-km与穗颈瘟的抗性呈正相关,相关系数分别0.81、0.11和0.15;稻瘟病抗性基因Pi-k^h与穗颈瘟抗性呈负相关,相关系数为-0.05。Rice blast is one of the major diseases of Japonica rice in Jiangsu province, and the neck blast is the most serious. Breeding resistant cultivars is an effective way to prevent this disease. In order to identify the primary resistance genes and genotypes of Japonica rice in Jiangsu province, functional markers of blast resistance genes Pi-ta, Pi-b, Pi-kh and Pi-km were used to detect 65 Japonica rice cultivars approved by Jiangsu province from 2007 to 2013. Six cultivars harbouring three rice blast resistance＆nbsp;genes, 23 cultivars harbouring two genes, 27 cultivars harbouring one gene, and 9 cultivars harbouring no gene were identi-fied, accounting for 9？2%, 35？4%, 41？5% and 13？9% respectively. All six cultivars harbouring three blast resistance genes performed resistance to neck blast. 19 out of 23 rice cultivars harbouring two resistance genes were resistant to neck blast. 12 out of 27 rice cultivars harbouring one resistance gene were resistant and the rest 15 were susceptible. Only 1 out of 4 cultivars without blast ristance gene showed neck blast resistance. Correlation and regression analyses revealed that Pi-ta, Pi-b and Pi-km were positively correlated with neck blast resistance of Japonica rice in Jiangsu province, and the corre-lation coefficients were 0？81, 0？11 and 0？15 respectively. Pi-kh was negatively correlated with neck blast resistance, with the correlation coefficient being -0？05.

关 键 词：粳稻 稻瘟病 穗颈瘟 稻瘟病抗病基因 

分 类 号：S511.220.34[农业科学—作物学]