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作 者:梁郸娜[1] 胡其靖[1] 曹磊[1] 宋琳琳[1] 徐强[1] 齐晓花[1] 周福才[1] 陈学好[1]
机构地区:[1]扬州大学园艺与植物保护学院,江苏扬州225009
出 处:《江苏农业学报》2016年第2期278-284,共7页Jiangsu Journal of Agricultural Sciences
基 金:国家重点基础研究发展计划(973计划)项目(2012CB113900)
摘 要:为了研究黄瓜抗蚜性与丙二醛(MDA)含量及保护酶活性的关系,选用抗蚜黄瓜品系EP6392和感蚜品系JY30为试验材料,在第1片真叶展平时,在每株的第1片真叶背面接种5头无翅成蚜作为处理,不接蚜虫的植株作为对照,测定黄瓜叶片中MDA含量和保护酶活性。结果表明:蚜虫接种后两品系的MDA含量和过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)的活性显著高于对照。感蚜品系的MDA含量始终极显著高于抗蚜品系;蚜虫侵染后,抗蚜品系POD活性较感蚜品系上升迅速,抗蚜品系PAL的活性极显著高于感蚜品系。因此,MDA、POD和PAL参与了黄瓜对蚜虫侵染的防御调控。To analyse the defensive role of MDA and protective enzymes against aphid in cucumber, cucumber lines EP6392 ( resistant) and JY30 ( susceptible) were measured for their MDA content and protective enzymes activities. Five apterous adult aphids were transferred to the back of the first true leaf of each cucumber plant as treatment and the seedling without aphids was set as control. After the inoculation, MDA content, and peroxidase ( POD) , phenylalanine ammonial-yase ( PAL) activities in cucumber seedlings were boosted in both lines. The MDA content in the susceptible line was signif-icantly higher than that in the resistant line. POD activity of the resistant line was increased more rapidly than that in sus-ceptible line. PAL activity in the resistant line was much higher than that in susceptible line after aphid infection. Therefore, MDA, POD and PAL may participate in the cucumber defense regulation against aphid infection.
分 类 号:S436.421.21[农业科学—农业昆虫与害虫防治]
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