凤眼莲对铜绿微囊藻生理、细胞结构及藻毒素释放与削减的影响  

Physiological characteristics and cell structure of Microcystis aeruginosa and microcystin release and reduction in Eichhornia crassipes-grown water

作　　者：杨小杰[1,2] 韩士群[2] 唐婉莹[1] 严少华[2] 周庆[2] 

机构地区：[1]南京理工大学化工学院,江苏南京210094 [2]江苏省农业科学院农业资源与环境研究所,江苏南京210014

出　　处：《江苏农业学报》2016年第2期376-382,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2015BAD13B04);江苏省自然科学基金项目(BK20130730)

摘　　要：通过共培养试验,研究了凤眼莲对产毒铜绿微囊藻生长和毒素释放的影响。结果表明,凤眼莲与铜绿微囊藻共培养有效抑制了铜绿微囊藻的生长,加速了它的衰亡。凤眼莲严重破坏藻细胞的超氧化物歧化酶(SOD)系统,共培养6 d后藻细胞SOD活性降至(2.67±1.68)U/mg,导致超氧阴离子自由基未能及时转化。在凤眼莲影响下,共培养4 d时铜绿微囊藻细胞就出现萎缩现象,类囊体片层结构出现溶解,细胞ATP水平持续快速下降。与无凤眼莲空白对照相比,凤眼莲的存在使铜绿微囊藻毒素(MC-LR和MC-RR)的释放量显著降低,削减速度显著加快。The influence of co-cultured Eichhornia crassipes on physiological characteristics and cell structure of Microcystic aeruginosa and different types of microcystins release and reduction were studied. E. crassipes inhibited the growth of M. aeruginosa and accelerated its death. E. crassipes also damaged the superoxide dismutase （ SOD） enzyme sys-tem of M. aeruginosa severely;the SOD activity of co-cultured algal cells decreased to （ 2？67 ± 1？68） U/mg after 6 d, which resulted in the failure of timely transformation of superoxide anion radical. Under the stress of E. crassipes for 4 d, cells of M. aeruginosa started to shrink, the thylakoid lamella structure was dissolved, and the ATP level of algal cells de-clined rapidly. However, the releases of microcystin-LR and microcystin-RR from M. aeruginosa in the existence of E. cras-sipes was significantly less, and the reductions was quicker.

关 键 词：凤眼莲 铜绿微囊藻 藻毒素释放 藻毒素削减 

分 类 号：X52[环境科学与工程—环境工程]