免疫增强剂提高H9亚型禽流感灭活疫苗在SPF鸡体中细胞因子的免疫应答  

Immunopotentiators improving the cytokine immune response to inactivated H9 subtype avian influenza vaccine in SPF chickens

作　　者：陆吉虎[1,2,3] 吴培培[1,3] 张雪花[1,2,3] 侯继波[1,3] 唐应华[1,3] 

机构地区：[1]江苏省农业科学院国家兽用生物制品工程技术研究中心/农业部兽用生物制品工程技术重点实验室,江苏南京210014 [2]南京天邦生物科技有限公司,江苏南京211102 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《江苏农业学报》2016年第2期408-413,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31201904);农业部公益性行业(农业)科研专项(201303046);中央财政农业技术推广资金项目[TG(14)067];江苏省农业科技自主创新基金项目[CX(14)5045]

摘　　要：为探讨免疫增强剂(VA5)提高SPF鸡免疫功能的机理,将SPF鸡分别免疫H9亚型禽流感灭活疫苗、含VA5的H9灭活疫苗、含IL-4和/或IFN-γ真核质粒的H9亚型禽流感灭活疫苗,另设空白对照。检测免疫鸡外周血CD4^+、CD8^+T淋巴细胞亚群数目及IL-4和IFN-γ细胞因子含量的变化及血清抗体效价。结果显示,在14日龄、17日龄和21日龄和28日龄,免疫IL-4和H9、IL-4与IFN-γ和H9以及VA5佐剂与H9组的CD4^+淋巴细胞亚群上升快且比例高于其他组,免疫IFN-γ和H9、IL-4与IFN-γ和H9以及VA5增强剂与H9组的CD8^+淋巴细胞亚群上升快且比例高于其他组,IL-4的上升趋势与CD4^+细胞类似,IFN-γ的上升趋势与CD8^+类似。免疫含VA5的禽流感(H9亚型)灭活苗组在免疫后第2周、3周和4周血清HI效价达到7lg2以上,高于其他各免疫组。说明,与直接添加IL-4和IFN-γ的细胞因子质粒组相比,VA5免疫增强剂能够促进SPF鸡外周血淋巴细胞增值,提高CD4^+、CD8^+T淋巴细胞的比例,并诱导IL-4和IFN-γ细胞因子分泌。The aim of this study is to explore the immunological mechanism of immunopotentiator VA5 in improving the immune response of specific pathogen-free （ SPF ） chickens. SPF chickens were challenged with the inactivated H9 subtype vaccine, VA5＋H9 vaccine, IL？4＋H9 vaccine, IFN？γ＋ H9 vaccine, IL？4 ＋ IFN？γ ＋ H9 vaccine, and blank control, respectively. The number of CD4＋ or CD8＋subgroup lymphocytes from peripheral blood mononuclear cells, the contents of IL？4 and IFN？γ from sera and the antibody levels of hemagglutinin inhibition （ HI） were detected. The number of CD4＋ subgroup lym？ phocytes from chickens in groups of IL？4＋H9, IL？4＋IFN？γ＋H9, and VA5＋H9 climbed faster and the percentage was higher than those in other groups at the 14？, 17？, 21？ and 28？day ages. The number of CD8＋ subgroup lymphocytes from chickens in groups of IFN？γ＋H9, IL？4 and IFN？γ＋H9 vaccine and VA5＋H9 vaccine climbedfaster and the percentage was higher than those in other groups IL4 showed similar rising trend to the CD4＋ subgroup lymphocytes and IFN？γ presented similar rising trend to the CD8＋subgroup lymphocytes. The HI antibody levels of chickens in group of VA5＋H9 were 7lg2 at 2？, 3？and 4？week postvaccination, higher than those in other groups. In summary, the immunopotentiator VA5 increased the percantages of CD4＋ and CD8＋ subgroup lymphocytes and induced high secretions of IL？4 and IFN？γ.

关 键 词：免疫增强剂 CD4+ T淋巴细胞 CD8+T淋巴细胞 细胞因子 血凝抑制抗体 

分 类 号：S831.7[农业科学—畜牧学]