杜鹃花种质资源遗传多样性的SRAP分析  

SRAP analysis of genetic relationships among Rhododendron species

作　　者：肖政[1] 苏家乐[1] 刘晓青[1] 李畅[1] 何丽斯[1] 陈尚平[1] 

机构地区：[1]江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014

出　　处：《江苏农业学报》2016年第2期442-447,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2013BAD01B070403);江苏省自然科学基金项目(BK20150548;BK2012789);江苏省农业科技自主创新项目[CX(13)2016]

摘　　要：采用SRAP分子标记分析了30个杜鹃花物种的遗传多样性。20对引物共检测到306个位点,平均每对引物扩增出15.3个位点,多态位点百分率为100%。物种水平的Nei’s基因多样性指数(H)为0.352,Shannon’s信息指数(I)为0.525,相似系数在0.543至0.761之间,表明参试的30份杜鹃花材料具有较高的遗传多样性。主坐标分析(PCA)结果和UPGMA法构建的系统树显示,30份杜鹃花材料可分为5类,其中云锦杜鹃和光枝杜鹃、井岗山杜鹃和皱叶杜鹃、迎红杜鹃和兴安杜鹃、露珠杜鹃和大果杜鹃分别聚为一组,结果与基于表型特征的分类基本一致,表明SRAP标记具有较准确的鉴别能力,是进行杜鹃花属植物遗传多样性分析的有效分子标记。Genetic diversity across 30 species of Rhododendron was analyzed using SRAP molecular markers. A total of 306 polymorphic bands were generated using 20 pairs of SRAP primers and the polymorphism percentage was 100%. Each pair of primers generated 15？3 bands on average. At the species level, Nei’ s gene diversity （ H） was 0？352, Shan-non’ s information index （ I） was 0？525 and the similarity coefficient ranged from 0？543 to 0？761, indicative of a high ge-netic diversity among 30 species. Principal component analysis and the unweighted pair group method with arithmetic mean （ UPGMA） cluster analysis reveal that the 30 species were divided into 5 groups. R. fortunei Lindl. and R. haoful Chun et Fang, R. jinggangshanicum Tam and R. denudatum Levl., R. mucronulatum Turcz. and R. dauricum L., R. irroratum Franch and R. sinonuttallii Balf. f. et Forrest were clustered together, respectively, consistent with the classification based on phenotypic characteristic. The result suggests that the SRAP is an effective molecular marker to analyze the Rhododen-dron genetic diversity.

关 键 词：杜鹃花属 序列相关扩增多态性 遗传多样性 主坐标分析 

分 类 号：S759.95[农业科学—森林经理学]