桑菊饮抗炎活性成分筛选与单体验证  

Screening of activity components with anti-inflammation in Sang Ju Yin and verification of monomer

作　　者：潘梓烨 常念伟 周梦鸽[2] 段茜茜 李延美[2] 姜民[1] 白钢[1] 

机构地区：[1]天津中医药大学,天津300073 [2]南开大学药学院,天津市分子药物研究重点实验室,天津300071

出　　处：《中草药》2016年第8期1289-1296,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81473403)

摘　　要：目的探究桑菊饮的抗炎活性,明确其抗炎活性物质基础;为进一步阐明桑菊饮抗炎作用机制,建立其质量标准提供依据。方法采用UPLC-Q/TOF技术结合核因子-κB(NF-κB)荧光素酶报告基因检测系统筛选桑菊饮中具有抗炎活性的药效成分,并选取人支气管上皮细胞(BEAS-2B),建立炎症损伤模型,对咖啡酸、绿原酸、甘草酸、马钱子苷和胡萝卜酸等成分的抗炎活性进行验证。结果筛选出16种具有抑制NF-κB活性的成分,其中胡萝卜酸、22β-乙酰基-甘草酸、甘草皂苷G2抑制NF-κB活性为首次报道。结论桑菊饮可以通过抑制NF-κB的表达发挥抗炎作用,其抗炎活性主要与16种单体物质相关。Objective To investigate the anti-inflammatory activity of Sang Ju Yin（Mulberry Leaf and Chrysanthemum Decoction）, and elucidate its bioactive components on nuclear factor-κappa B（NF-κB） inhibition, and to further clarify the anti-inflammatory mechanism of Sang Ju Yin, so as to provide the basis for establishing its quality standard. Methods UPLC-Q/TOF MS coupled with NF-κB activity luciferase reporter assay system was applied to determine the potential anti-inflammatory components in Sang Ju Yin. And the human bronchial epithelial cells（BEAS-2B） were selected to set up the inflammatory injury model, caffeic acid, chlorogenic acid, glycyrrhizic acid, loganin, and malonic acid in Sang Ju Yin were investigated for their anti-inflammation. Results Sixteen components were screened to have the NF-κB inhibitory effects. Among them, malonic acid, 22β-acetoxyl-glycyrrhizic acid, and licorice saponin G2 were first reported to have the NF-κB inhibitory activity. Conclusion Sang Ju Yin contains the potential NF-κB inhibitors. Sixteen potentially active ingredients are found to have the anti-inflammatory effects as NF-κB inhibitors.

关 键 词：桑菊饮 抗炎 UPLC-Q/TOF 核因子-κB(NF-κB) 药效物质基础 

分 类 号：R284[医药卫生—中药学] R284.51[医药卫生—中医学]