大口黑鲈ghrelin基因SNPs的筛选及与生长性状关联性分析  

SNPs detection of ghrelin gene and its association with growth traits in largemouth bass(Micropterus salmoides)

作　　者：刘浩[1,2] 白俊杰[1,2] 李胜杰[1] 樊佳佳[1] 全迎春[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《水产学报》2016年第4期521-527,共7页Journal of Fisheries of China

基　　金：国家科技支撑计划(2012BAD26B03);国家自然科学基金(31201985);“九四八”计划重点项目(2011-G12)~~

摘　　要：ghrelin是脊椎动物的一种脑肠肽,有促进摄食的功能,并能促进生长激素(GH)释放,参与能量平衡调控和糖类代谢。为探索ghrelin基因多态性与大口黑鲈生长性状的相关性,针对大口黑鲈ghrelin基因的启动子序列,实验采用直接测序法获得了2个SNPs位点:S1(A-642C)和S2(A-639C)。随机选取同批繁殖、同塘养殖的大口黑鲈采用Sna Pshot方法进行SNPs位点检测和分型。结果显示,实验群体在ghrelin基因2个SNPs位点上基本处于哈温平衡,S1和S2共组成5种双倍型(D1、D2、D3、D4和D5)。基因型与生长性状的相关性分析结果表明,S1位点AC型个体在体高与全长上显著高于CC型个体,S2位点AA型个体在头长上显著高于AC型个体。双倍型D1在体质量、全长和体高方面显著高于双倍型D3,在体质量、体宽和体高方面显著高于双倍型D4。本研究在大口黑鲈ghrelin基因启动子区域获得的与生长性状相关的SNPs标记,可为大口黑鲈分子标记辅助育种提供帮助。Ghrelin is one of the vital brain-gut peptides in vertebrates. In 1999, a noval 28-amino acid gut-brain peptide named ghrelin was first isolated from the mammalian stomach. Similar to the synthetic molecules of growth hormone secretagogues, ghrelin was proved to be an endogenous ligand for growth hormone secretagogue receptor and involved in growth hormone secretion, food intake, carbohydrate metabolism, and energy homeostasis. So, ghrelin gene is a potential candidate gene for fish growth traits. This study detected the polymorphism of the promoter of largemouth bass （Micropterus salmoides） ghrelin gene by DNA sequencing in 327 individuals, for exploring the association between the polymorphism of ghrelin and growth traits of largemouth bass. Two single nucleotide polymorphism （SNP） mutations were identified （S1：A-642C, S2：A-639C） of the gene. The genotype and gene frequency of 327 largemouth bass were further assayed with the method of SnaPshot, at site S1, there three kinds of genotypes named AA, AC and CC, the gene frequency were 0.067, 0.685 and 0.248 respectively. There were only two genotypes AA and AC on the locus of S2, of which gene frequency were 0.850 and 0.150 respectively. Genetic structure was analyzed by POPGENE32 software, the results showed that the two locus were in Hardy-Weinberg equilibrium, the effective number, expected heterozygosity and observed heterozygosity of S1 were 1.9362, 0.4843 and 0.6831 respectively, which of S2 were 1.1620, 0.1396 and 0.1508. The sites of S1 and S2 formed five diplotypes（D1, D2, D3, D4 and D5）. Association analysis showed that individuals with genotype AC were significantly higher than those of individuals with genotype CC in body depth and total length at site S1. At site S2, the individuals of genotype with AA significantly showed a higher value on head length compared to the genotype of AC. The five diplotypes consisted of the two SNPs were different on some growth traits, the body weight, total length and body depth of diplotypes D1 show

关 键 词：大口黑鲈 GHRELIN基因 SNPS 双倍型 生长 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]