精浆中人附睾蛋白4与男性不育症的相关性  

Correlation between human epididymis protein 4 in seminal plasma and male infertility

作　　者：席向红[1] 苏荣[1] 贾韶彤[2] 唐秀英[1] 王利新[1] 

机构地区：[1]宁夏医科大学总医院医学实验中心,宁夏银川750004 [2]宁夏医科大学总医院生殖医学中心,宁夏银川750004

出　　处：《山东大学学报（医学版）》2016年第5期66-69,共4页Journal of Shandong University:Health Sciences

基　　金：宁夏自治区卫计委重点科研计划课题(2014-NW-021)

摘　　要：目的分析精浆中人附睾蛋白4(HE4)含量与男性不育症的相关性,探讨精浆HE4检测在男性不育症诊断中的应用价值。方法采集231例生殖男科就诊的不育症患者精液,依据WHO诊断标准分为5组:无精子症组45例、少精子症组43例、弱精子症组41例、畸形精子症组45例和精液参数正常组57例,选取40例已生育者为正常生育组,采用计算机辅助精子分析仪(CASA)分析各组精液精子浓度、精子总数、精子活力、前向运动精子(PR)和精子形态,电化学发光法检测各组精浆中HE4含量,并与精液常规指标进行相关性分析。结果 271例男性精浆HE4平均含量为(793.4±362)pmol/L。无精子症、少精子症、弱精子症和畸形精子症组精浆中HE4含量分别为(987.43±386.75)、(857.12±355.64)、(807.72±375.21)和(837.04±371.36)pmol/L,均高于正常生育组(604.45±272.02)pmol/L,P值分别为0.000、0.001、0.009、0.002。少精子症、弱精子症和畸形精子症组中精子浓度、精子总活力和PR均低于正常生育组(P值均为0.000),且精浆HE4含量与精子浓度、精子总数、精子总活力、PR及正常形态精子百分率均呈负相关(r值分别为-0.371、-0.348、-0.284、-0.240、-0.158)。结论精浆HE4含量与男性不育症患者精液常规五项指标呈负相关,可作为判断男性生育能力的参考指标。Objective To analyze the relationship between human epididymis protein 4（ HE4） content in seminal plasma and male infertility. Methods According to the WHO diagnostic standard,we collected semen from 231 infertile patients and divided them into 5 groups： azoospermia group（ n = 45）,oligozoospermia group（ n = 43）,asthenozoospermia group（ n = 41）,teratozoospermia group（ n = 45） and normal group（ n = 57）. Another 40 controls who had normal fertility was enrolled as the control group. The sperm density,total sperm count,sperm motility,forward movement,and sperm morphology were analyzed with computer-aided sperm analysis（ CASA）. The HE4 content was detected with electrochemical luminescence. Then the correlation between routine sperm parameters and HE4 content was analyzed. Results The average HE4 content in 271 cases of seminal plasma was（ 793. 4 ± 362） pmol / L. The HE4 content in the azoospermia,oligozoospermia,asthenozoospermia,and teratozoospermia groups was（ 987. 43 ± 386. 75）,（ 857. 12 ± 355. 64）,（ 807. 72 ± 375. 21） and（ 837. 04 ± 371. 36） pmol/L,respectively,which was higher than that of the normal group（ 604. 45 ± 272. 02） pmol / L,P = 0. 000,0. 001,0. 009,0. 002,respectively）. The sperm density,motility and PR in the azoospermia,oligozoospermia,asthenozoospermia,and teratozoospermia groups were significantly lower than in the normal fertility group（ P = 0. 000）. The HE4 content in seminal plasma was negatively correlated with sperm density,total sperm count,sperm motility,PR and normal morphology（ r =- 0. 371、- 0. 348、- 0. 284、- 0. 240、- 0. 158）. Conclusion The HE4 content in seminal plasma was negatively correlated with the routine sperm parameters,indicating that it can serve as a reference index of male fertility.

关 键 词：精浆 人附睾蛋白4 不育症 精液常规参数 

分 类 号：R698.2[医药卫生—泌尿科学]