番石榴叶总三萜改善糖尿病周围神经病变  

Ameliorative effect of total triterpenoids from Psidium guajava leaves on diabetic peripheral neuropathy

作　　者：叶开和[1] 王小康[1] 吕艳青[1] 魏崧丞 李秀存[1] 张晓琦[2] 叶春玲[1] 

机构地区：[1]暨南大学药学院药理学教研室,广东广州510632 [2]暨南大学中药及天然药物研究所,广东广州510632

出　　处：《中成药》2016年第5期961-966,共6页Chinese Traditional Patent Medicine

基　　金：广东省高等学校科技创新重点项目(cxzd1111);国家自然科学基金(81373935);广东省重大科技专项(2013A022100028)

摘　　要：目的观察番石榴叶总三萜(TTPGL)对糖尿病大鼠周围神经病变(DPN)的改善作用,并探讨其作用机制。方法采用高脂饮食加腹腔注射链脲佐菌素(STZ)建立糖尿病大鼠模型。将糖尿病大鼠随机分为6组,石榴叶总三萜低、中、高剂量组[30、60、120 mg/(kg·d)],正常组,模型组和罗格列酮组。测定坐骨神经运动传导速度(MCV)并对组织HE染色;利用RT-PCR检测坐骨神经组织肿瘤坏死因子-α(Tnfa),白细胞介素-6(Il6)和一氧化氮合酶(i NOS)mRNA的表达量;印迹法检测蛋白激酶B(AKT)、核转录因子κB激酶抑制因子IKKα、p65及其各自磷酸化蛋白表达。结果与模型组比较,石榴叶总三萜(120 mg/kg)组显著地改善了坐骨神经的神经病变(P<0.05);石榴叶总三萜显著性抑制各炎症因子mRNA的表达(P<0.05),并显著下调p-AKT/AKT、p-IKKα/IKKα、p-p65/p65的表达(P<0.01或P<0.05)。结论石榴叶总三萜具有显著改善DPN的作用,其机制可能通过下调NF-κB介导的信号通路,从而减少炎症介质产生。AIM To investigate the ameliorative effect of total triterpenoids from Psidium guajava leaves（ TTPGL） on diabetic peripheral neuropathy（ DPN） in rats and to explore the underlying mechanism.METHODS Rats models for diabetic were induced by high-fat feeding and STZ injection. These rats were randomized into six groups,TTPGL-30,60 and 120 groups [30,60 and 120 mg /（ kg · d） ],control group,model group and positive control rosiglitazone group [3 mg /（ kg · d） ]. Nerve conduction velocity（ MCV） and histopathological staining were performed on sciatic nerves. mRNA expressions of pro-inammatory molecules（ Tnfa,Il6 and i NOS） and tissue proteins（ AKT,IKKα and p65） were also estimated by RT-PCR and Western blot,respectively. RESULTS Compared with the model group,TTPGL（ 120 mg / kg） group significantly ameliorated DPN nerve deficits（ P〈0. 05）. It significantly inhibited Tnfa, Il6 and iNOS mRNA expressions（ P〈0. 05）,and significantly down-regulated p-AKT/AKT,p-IKKα /IKKα and p-p65 /p65（ P〈0. 01 or P〈0. 05）. CONCLUSION TTPGL can markedly improve DPN symptom,whose mechanism may be related to the down-regulation of NF-κB-mediated signal pathway,and then decrease the expressions of inflammatory mediators.

关 键 词：番石榴叶总三萜 糖尿病周围神经病变 炎性介质 NF-ΚB通路 

分 类 号：R285.5[医药卫生—中药学]