猪PR39真核表达载体构建  

Construction of eukaryotic expression vector of porcine PR39

作　　者：江学斌[1] 陈嘉蔚[2] 杨军[2] 胡位荣[1] 肖安吉 卢志鹏[2] 楚品品 张玲华[2] 

机构地区：[1]广州大学生物工程研究所,广东广州510006 [2]华南农业大学生命科学学院/广东省农业生物蛋白质功能与调控重点实验室,广东广州510642

出　　处：《广东农业科学》2016年第3期148-151,共4页Guangdong Agricultural Sciences

基　　金：广州市属高校科技计划项目(12014 20755)

摘　　要：为构建猪源抗菌肽PR39的高效表达真核载体pVAX1-PR39,通过猪股骨骨髓组织基因组RNA合成c DNA,根据PR39基因全长序列,设计高效表达启动子,利用PCR扩增获得PR39全长基因,连接到真核载体pVAX1,构建重组载体pVAX1-PR39。转化大肠杆菌DH5α后进行菌落PCR鉴定和测序,测序结果与NCBI网站上猪源抗菌肽PR39基因序列完全一致,表明已成功构建了猪PR39的真核表达载体,理论上所构建的PR39表达载体能够提高猪的免疫能力。To construct the eukaryotic expression vector of porcine antimicrobial peptide PR39, cDNA was synthesized by the RNA from bone marrow of porcine femur. According to the full length sequence of PR39 gene, a efficient expression gene sequence was designed, and the full length gene of PR39 was amplified by PCR and connected to the eukaryotic vector pVAX1, then the recombinant vector pVAX1-PR39 was constructed and transferred into E. coli DH5 c~ followed by colony PCR identification and sequencing. The results showed that the sequences were completely consistent with the PR39 gene sequences published on NCBI. The PR39 eukaryotic expression vector was constructed and PR39 expression vector was capable of improving the immune ability of pig in theory.

关 键 词：抗菌肽 PR39 真核表达 载体 PVAX1 

分 类 号：S813-3[农业科学—畜牧学] S828[农业科学—畜牧兽医]