臭氧化盐水对大鼠切口痛模型行为学及血浆TNF-α、 IL-6的影响  

Influence of ozonized saline on behaviors of rat models with incisional pain and levels of plasma TNF-α and IL-6

作　　者：王雪梅[1] 王亚平[2] 吕志平[2] 江兴华[2] 邹定全[1] 

机构地区：[1]贵阳市妇幼保健院 [2]中南大学湘雅二医院麻醉科,长沙410011

出　　处：《中国医师杂志》2016年第4期508-511,共4页Journal of Chinese Physician

摘　　要：目的 观察静脉注射不同浓度臭氧化盐水对大鼠切口痛模型行为学及血浆TNF-α、IL-6水平的影响.方法 健康成年雄性SD大鼠54只,选取24只用于疼痛行为学测试,随机分为4组,每组6只,于造模前10 min经尾静脉给药5 ml/kg,A1组给氧气化盐水,B1、C1、D1组分别给20μg/ml、30 μg/ml、40 μg/ml的臭氧化盐水,七氟烷麻醉后制作切口痛模型,分别在术前24 h、术后2、6、24、48 h五个时间点测试大鼠50％机械缩足阈值（50％ PWMT）和累积疼痛评分（CPS）.其余30只随机分为五组,每组6只,A2、B2、C2、1D2组处理同前,E2组为假手术组,术后2h右心室取血2ml,用ELISA法检测大鼠血浆TNF-α和IL-6水平.结果 与A1组比较,B1组在术后各时间点50％PWMT差异无统计学意义（P＞0.05）,而C1、D1两组在术后2、6、24、48 h 50％ PWMT明显大于A1组,差异有统计学意义（P＜0.05）;与A1组比,B1、C1、D1组在术后不同时点CPS均小于A1组,差异有统计学意义（P＜0.01）.术后2h大鼠血浆TNF-α浓度E2组与B2、D2组差异无统计学意义（P＞0.05）,A2组和C2组较E2组升高,其中C2组高于A2组;术后2h各组大鼠血浆IL-6浓度E2组与A2组差异无统计学意义（P＞0.05）,B2、C2、D2组均较E2组升高,其中D2组最高,B2组最低.结论 臭氧化盐水静脉注射对大鼠切口痛有镇痛效果,且存在浓度相关性.Objective To investigate the analgesia effect and the possible mechanism of intravenous administration with different concentrations of ozonized saline （OS） by observing changes in behavior,plasma tumor necrosis alpha （TNF-α）,and interleukin-6 （IL-6） levels after rat plantar incision.Methods Fifty-four health adult male Sprague-Dawley rats were used in the investigation.Twenty four rats were randomly divided into four groups,6 in each group.The rats in group A1 were intravenously administered with 5ml/kg oxygen saline,10min before the incision.The rats in groups B1,C1,and D1 were intravenously administered with 20 μg/ml,30 μg/ml,and 40 μg/ml OS 5 ml/kg.An 1-cm incision was made in the right plantar surface from the heel to the toes according to the method described by Brennan under sevoflurane anesthesia.The 50% paw withdrawal mechanical threshold （50% PWMT） and cumulative pain score （CPS） at the times of 24 h before and 2,6,24,48 h after surgery were underwent.Thirty rats were randomly divided into five groups,6 in each group.Groups A2,B2,C2,and D2 processed as well as group 1.All groups except group E2 were made the model of incisional pain.2 ml blood was taken out of the right ventricular 2 h after the operation,then the levels of plasma TNF-α and IL-6 were detected by using enzyme linked immunosorbent assay （ELISA）.Results There were no significant differences in the 50%PWMT between group A1 and group B1 at each time point after surgery （P 〉 0.05）.The 50% PWMT in groups C1 and D1 were higher than those in group A1 at each time point after surgery （P 〈 0.05）.The CPS in groups B1,C1,and D1 were lower than those in group A1 after surgery （P 〈 0.01）.Compared to group E2,the levels of plasma TNF-α 2 h after the operation in group B2 and D2 were not statistically different （P 〉 0.05）.The levels of plasma TNF-α in groups C2 and A2 were higher than those in group E2 （P 〈 0.05）.The levels of plasma IL-6 2 h after the operation between group A2 and group E2 s

关 键 词：臭氧/药理学 疼痛 手术后/药物疗法/代谢 肿瘤坏死因子α/代谢 白细胞介素6/代谢 

分 类 号：R614[医药卫生—麻醉学] R-332[医药卫生—外科学]