拉莫三嗪对小鼠脊髓背侧半横断损伤的保护作用  

Protective effect of lamotrigine on mouse dorsal spinal cord injury

作　　者：付强 王兆涛 张茂营[2] 徐如祥[2] 

机构地区：[1]解放军医学院,北京100853 [2]北京军区总医院附属八一脑科医院,100700

出　　处：《中华神经创伤外科电子杂志》2015年第3期30-35,共6页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：军队十二五重大科技项目(BWS12J010)

摘　　要：目的探讨拉莫三嗪在小鼠脊髓损伤修复中的作用。方法雌性C57BL/6小鼠80只采用脊髓背侧半横断损伤模型,随机分为模型组：单纯脊髓损伤;治疗组：脊髓损伤后每天给予腹腔注射拉莫三嗪,按照25 mg/kg的计量,连续7 d;对照组：脊髓损伤后每天给予腹腔注射0.9%生理盐水,按照25 mg/kg的计量,连续7 d。术后分别在1 d、7 d、14 d对各组小鼠行BBB评分,并分别在1 d、7 d、14 d在各组随机抽取6只小鼠,进行免疫荧光染色观察胶质细胞变化,并在术后第7天各组取6只小鼠组织行ELISA检测,观察炎症因子表达情况。结果损伤后7、14 d治疗组小鼠BBB评分明显高于模型组和对照组,差异有统计学意义（P〈0.05）;脊髓损伤后矢状切面损伤灶下游的GFAp＋（星形胶质细胞的标记）细胞的免疫荧光面积百分比,发现术后7 d和14 d治疗组（9.87±0.96,4.79±1.02）的明显低于模型组（12.34±1.72,7.46±1.28）和对照组（11.89±1.70,7.53±1.70）,差异均有统计学意义（P〈0.05）。ELISA检测术后7 d小鼠T9~T11的IL-1、IL-10、TNF-α表达情况,IL-1、IL-10的表达治疗组明显低于模型组和对照组,差异有统计学意义（P〈0.05）。结论拉莫三嗪可以改善脊髓损伤小鼠的运动功能,通过前期减少炎症因子的分泌降低胶质细胞的活化来实现的。Objective To investigate the effect of lamotrigine （LTG） on recovery after spinal cord injury（SCI）. Methods A total of 80 female C57BL/6 mouses were made the model of spinal cord hemisection, and all of these were randomly divided into 3 group：SCIgroup,SCI＋LTG group and SCI＋0.9%saline group;mouses in SCI＋LTG grouop were given interperitoneal injection of lamotrigine （25 mg/kg） for 7days, and mouses in SCI＋0.9%saline group were given the same volume of 0.9%saline. Basso, Beatti, Bresnahan（BBB）scale was perfomed 1, 7, 14days after SCI;the number of glial cell were observed by immunofluorescence and the express of inflammatory factors were obseverd by ELISA （enzyme linked immunosorbent assay）. Results On the 7th, 14th days after injury, the BBB scale scores in the SCI＋LTG group were significantly higher than those in the SCI group and in the SCI＋0.9%saline group （P〈0.05）. On the 7th, 14th days after injury, less percentage of GFAP＋fluorenscent area and less number of Iba1＋cells in the SCI＋LTG group were distributed in the lesion area than those in the SCI group and in the SCI＋0.9%saline group （P〈0.05）. On the 7th day after spinal cord injury, the IL-1 and IL-10 expression in SCI＋LTG group were obviously lower than those in the SCI group and in the SCI＋0.9% saline group（P〈0.05）. Conclusion Lamotrigine improve the motor function after spinal cord injury by decrease the activation of the glial cells.

关 键 词：脊髓损伤 拉莫三嗪 胶质细胞 炎症因子 

分 类 号：R651.2[医药卫生—外科学]