缺氧预处理对颅脑外伤大鼠皮层TrxR2、CHOP表达及神经元细胞超微结构改变的影响  

Effects of hypoxic preconditioning on cortical expression of TrxR2 and CHOP of rats with craniocerebral injury and ultramicrostructural changes of neurons

作　　者：刘光杰[1] 刘家传[1] 王金标[1] 杨艳艳[1] 张星[1] 王春琳[1] 汤宏[1] 徐燊[1] 

机构地区：[1]解放军第105医院神经外科,合肥230031

出　　处：《中华神经创伤外科电子杂志》2015年第3期36-40,共5页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：全军医学科技"十二五"科研面上项目(编号:CWS11J262);2009年度南京军区医学科技创新重点课题(编号:09Z009)

摘　　要：目的探讨缺氧预处理对颅脑外伤大鼠皮层TrxR2、CHOP表达及神经元细胞超微结构改变的影响。方法 48只SD大鼠随机分成对照(Con)组、缺氧预处理(HPC)组、单纯外伤(TBI),组和缺氧预处理颅脑外伤(HPCT)组。采用改良的Feeney's自由落体装置制作颅脑损伤大鼠模型,预先在低压氧舱内连续处理3 d(-50 kPa、3 h/d)制作缺氧预处理模型。采用HE染色法和新鲜标本制作超薄切片,分别在光镜和电镜下观察各组大鼠皮层脑组织的大体结构和神经元细胞超微结构改变。qRT-PCR和Western blotting法检测挫伤区周围皮层TrxR2、CHOPmRNA和蛋白的表达变化。结果 Con组和HPC组病理改变无明显差异,HPCT组较TBI组大鼠病理改变轻,神经元细胞超微结构受损明显减轻。HPCT与TBI组比较,TrxR2 mRNA和蛋白表达显著上调,CHOPmRNA和蛋白表达显著下调,差异均具有统计学意义(均P<0.05);Con与HPC组比较,差异无统计学意义(P>0.05)。结论缺氧预处理可明显减轻颅脑外伤大鼠皮层神经元细胞形态学改变,这可能与上调TrxR2、下调CHOP的表达有密切关系,从而对神经元细胞起保护作用。Objective To investigate the effects of hypoxic preconditioning on the expression of TrxR2、CHOP and the ultra-structure of the neuronal cells of rats suffered traumatic brain injury. Methods Forty eight Sprague Dawley rats were randomly divided into control group （Con）, hypoxic preconditioning group （HPC）, traumatic brain injury group （TBI） and hypoxic preconditioning traumatic brain injury group （HPCT）. The traumatic brain injury model of rats were made by the Feeney’ s improved equipment and hypoxic preconditioning model were made by Hypobaric chamber for successive 3 days （-50 kPa、3 h/d）. Staining HE and making ultra-thin slicing by fresh specimen to observe the potential change of general structure and ultra-structure of rats’ brain tissue of each group respectively under light microscope and electron microscope. Expressions of TrxR2、CHOP mRNA and proteins were detected in the brain cortex around the contusion area by qRT-PCR and Western blotting. Results The pathologic change has no difference between Con gourp and HPC group. HPCT group has a lighter pathologic change than the TBI group.The expressions of TrxR2 mRNA and protein was significantly higher in the brain cortex in the HPCT group than the TBI group, the difference between the two group was significant （P〈0.05）, the expressions of CHOP mRNA and protein was significantlylower in the brain cortex in the HPCT group than the TBI group, the difference between the two group was significant （P〈0.05）, while the difference between the Con group and the HPC group was not significant （P〉0.05）. Conclusion Hypoxia preconditioning can obviously reduce the craniocerebral trauma rats cortex neuron cell morphology change,which has a close relationship with the upregulated expression of TrxR2 and the downregulated expression of CHOP, in order to protection of neurons.

关 键 词：颅脑外伤 缺氧预处理 TrxR2 CHOP 超微结构 

分 类 号：R651.15[医药卫生—外科学]