20AA复方氨基酸联用大剂量维生素B6治疗创伤凝血障碍大鼠的实验研究  

Effects of new therapy of 20 AA compound amino acid plus high dose of vitamin B6 on trauma-induced coagulopathy

作　　者：岳茂兴[1] 梁华平[2] 楚鹰[1] 包卿 郑旭文 刘政[2] 沈文明[1] 周培根[1] 李瑛[1] 卞晓星[1] 黄琴梅[1] 

机构地区：[1]江苏大学附属武进医院,常州213002 [2]第三军医大学创伤烧伤与复合伤国家重点实验室,重庆400042

出　　处：《中华卫生应急电子杂志》2015年第2期11-14,共4页Chinese Journal of Hygiene Rescue(Electronic Edition)

基　　金：第三军医大学国家重点实验室开放课题（SKLKF201312）;江苏省卫生厅重大课题（Z201013）;苏卫科教[2010]11号;常州市2014年度基础应用研究项目编号（CJ20140001）;

摘　　要：目的：探讨20AA 复方氨基酸注射液（丰诺安）＋大剂量维生素 B6新疗法对创伤性凝血功能障碍动物模型凝血功能的影响。方法实验动物为雄性 SD 鼠24只，随机分为假手术组、生理盐水组以及新疗法治疗组，每组8只。采用 Feeney′s 自由落体硬膜外撞击法加大腿骨折与放血制作大鼠多发伤模型，颈静脉插管输注20AA 复方氨基酸注射液（丰诺安）＋大剂量维生素 B6，生理盐水组输注等量的生理盐水，24 h 后，检测大鼠的凝血功能变化。结果治疗后新疗法治疗组与生理盐水组比较，血栓弹力图 R 值新疗法治疗组[（2．24±0．68）min]显著低于生理盐水组[（3．21±0．30）min]，（t ＝－0．98，P ＜0．05）；血栓弹力图 Angle 值新疗法治疗组[（81．98±1．78）deg]显著高于生理盐水组[（79．54±2．13）deg]，（t ＝2．43，P ＜0．05）。传统凝血试验结果：凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、凝血酶时间（TT）新疗法治疗组均比生理盐水组低，而纤维蛋白原（FIB）新疗法治疗组高于生理盐水组，但其差异不显著。结论20AA 复方氨基酸注射液（丰诺安）＋维生素 B6联用能够明显改善创伤后大鼠凝血功能。Objective To establish an animal model of coagulopathy,and to observe the effect of new therapy of 20AA compound amino acid injection plus high-dose vitamin B6 on coagulation function in the animal model.Methods The male SD rats were randomly divided into shame group,saline group and new therapy group,eight each group.The multiple trauma model of rat was established by using Feeney＇s free fall epidural impact method,plus bilateral femoral fractures and bleeding.The new therapy group was infused with 20AA compound amino acid injection plus high dose of vitamin B6 by jugular vein intubation.The control group was infused with same volume of saline injection.The changes of coagulation function in rats were investigated after 24 hours.Results The R value of thrombelastography in the new therapy group [（2.24 ±0.68）min]was significantly lower than that of the control group [（3.21 ±0.30）min）],（t =-0.98,P 〈0.05 ）.The Angle value of thrombelastography in the new therapy group [（81.98 ± 1.78）deg]was significantly higher than that of the control group [（79.54 ±2.13）deg],（t =2.43,P 〈0.05）.In traditional coagulation test results,the prothrombin time （PT）,activated partial clotting enzyme live time （APTT）,thrombin time （TT）in the new therapy group were lower than the values in the control group,but fibrinogen （FIB）was higher than the one of the control group,but the differences were not＆amp;nbsp;statically significant.Conclusion The treatment of 20AA compound amino acid injection plus high dose of vitamin B6 can significantly improve the coagulation function in the rat model of multiple trauma.

关 键 词：创伤和损伤 凝血酶 氨基酸类 大鼠 Sprague—dawley 

分 类 号：R641[医药卫生—外科学]